在患有和未患断奶后多系统消耗综合征的猪的血清和组织样本中检测1型和2型猪圆环病毒
Detection of porcine circovirus types 1 and 2 in serum and tissue samples of pigs with and without postweaning multisystemic wasting syndrome.
作者信息
Calsamiglia Maria, Segalés Joaquim, Quintana Josefina, Rosell Carles, Domingo Mariano
机构信息
Centre de Recerca en Sanitat Animal (CReSA), Campus Universitari Bellaterra, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain.
出版信息
J Clin Microbiol. 2002 May;40(5):1848-50. doi: 10.1128/JCM.40.5.1848-1850.2002.
Abstract
Presence of porcine circovirus type 1 (PCV1) and PCV2 was studied in sera and superficial inguinal lymph nodes from postweaning multisystemic wasting syndrome (PMWS)-affected and non-PMWS-affected pigs by using in situ hybridization and PCR. PCV1 and PCV2 were found in less than 3% and more than 50% of the samples, respectively. The most sensitive technique and site was PCR in superficial inguinal lymph nodes, but in situ hybridization correlated better with presence of characteristic lesions.
摘要
采用原位杂交和聚合酶链反应（PCR）技术，对受断奶后多系统消耗综合征（PMWS）影响和未受影响的猪的血清及腹股沟浅表淋巴结中猪圆环病毒1型（PCV1）和猪圆环病毒2型（PCV2）的存在情况进行了研究。分别在不到3%和超过50%的样本中发现了PCV1和PCV2。最敏感的技术和部位是腹股沟浅表淋巴结的PCR，但原位杂交与特征性病变的存在相关性更好。
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1
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Vet Rec. 2001 Sep 22;149(12):357-61. doi: 10.1136/vr.149.12.357.
2
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J Clin Microbiol. 2000 Sep;38(9):3474-7. doi: 10.1128/JCM.38.9.3474-3477.2000.
3
Serum antibodies to porcine circovirus type 1 and type 2 in pigs with and without PMWS.患有和未患仔猪多系统衰竭综合征（PMWS）的猪体内针对1型和2型猪圆环病毒的血清抗体
Vet Rec. 2000 Jun 24;146(26):762-4. doi: 10.1136/vr.146.26.762.
4
Genetic characterization of type 2 porcine circovirus (PCV-2) from pigs with postweaning multisystemic wasting syndrome in different geographic regions of North America and development of a differential PCR-restriction fragment length polymorphism assay to detect and differentiate between infections with PCV-1 and PCV-2.来自北美不同地理区域患断奶后多系统消耗综合征猪的2型猪圆环病毒（PCV - 2）的基因特征分析以及用于检测和区分PCV - 1与PCV - 2感染的差异聚合酶链反应-限制性片段长度多态性检测方法的建立
J Clin Microbiol. 2000 Jul;38(7):2494-503. doi: 10.1128/JCM.38.7.2494-2503.2000.
5
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Vet Microbiol. 2000 Jul 3;75(1):83-9. doi: 10.1016/s0378-1135(00)00199-1.
6
Porcine circoviruses: a review.猪圆环病毒：综述
J Vet Diagn Invest. 2000 Jan;12(1):3-14. doi: 10.1177/104063870001200102.
7
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Vet Rec. 1999 Jul 31;145(5):140-2. doi: 10.1136/vr.145.5.140.
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J Virol Methods. 1999 Jun;80(1):69-75. doi: 10.1016/s0166-0934(99)00032-4.
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