Calsamiglia Maria, Segalés Joaquim, Quintana Josefina, Rosell Carles, Domingo Mariano
Centre de Recerca en Sanitat Animal (CReSA), Campus Universitari Bellaterra, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain.
J Clin Microbiol. 2002 May;40(5):1848-50. doi: 10.1128/JCM.40.5.1848-1850.2002.
Presence of porcine circovirus type 1 (PCV1) and PCV2 was studied in sera and superficial inguinal lymph nodes from postweaning multisystemic wasting syndrome (PMWS)-affected and non-PMWS-affected pigs by using in situ hybridization and PCR. PCV1 and PCV2 were found in less than 3% and more than 50% of the samples, respectively. The most sensitive technique and site was PCR in superficial inguinal lymph nodes, but in situ hybridization correlated better with presence of characteristic lesions.
采用原位杂交和聚合酶链反应（PCR）技术，对受断奶后多系统消耗综合征（PMWS）影响和未受影响的猪的血清及腹股沟浅表淋巴结中猪圆环病毒1型（PCV1）和猪圆环病毒2型（PCV2）的存在情况进行了研究。分别在不到3%和超过50%的样本中发现了PCV1和PCV2。最敏感的技术和部位是腹股沟浅表淋巴结的PCR，但原位杂交与特征性病变的存在相关性更好。
