果蝇的全基因组组装
A whole-genome assembly of Drosophila.
作者信息
Myers E W, Sutton G G, Delcher A L, Dew I M, Fasulo D P, Flanigan M J, Kravitz S A, Mobarry C M, Reinert K H, Remington K A, Anson E L, Bolanos R A, Chou H H, Jordan C M, Halpern A L, Lonardi S, Beasley E M, Brandon R C, Chen L, Dunn P J, Lai Z, Liang Y, Nusskern D R, Zhan M, Zhang Q, Zheng X, Rubin G M, Adams M D, Venter J C
机构信息
Celera Genomics, Inc., 45 West Gude Drive, Rockville, MD 20850, USA.
出版信息
Science. 2000 Mar 24;287(5461):2196-204. doi: 10.1126/science.287.5461.2196.
We report on the quality of a whole-genome assembly of Drosophila melanogaster and the nature of the computer algorithms that accomplished it. Three independent external data sources essentially agree with and support the assembly's sequence and ordering of contigs across the euchromatic portion of the genome. In addition, there are isolated contigs that we believe represent nonrepetitive pockets within the heterochromatin of the centromeres. Comparison with a previously sequenced 2.9- megabase region indicates that sequencing accuracy within nonrepetitive segments is greater than 99. 99% without manual curation. As such, this initial reconstruction of the Drosophila sequence should be of substantial value to the scientific community.
我们报告了黑腹果蝇全基因组组装的质量以及完成该组装的计算机算法的性质。三个独立的外部数据源基本上认同并支持该组装的基因组常染色质部分的序列和重叠群排序。此外，存在一些孤立的重叠群，我们认为它们代表着着丝粒异染色质中的非重复区域。与先前测序的2.9兆碱基区域相比，表明在无需人工编辑的情况下，非重复片段内的测序准确率大于99.99%。因此，果蝇序列的这一初步重建对科学界应具有重大价值。
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