黑腹果蝇异染色质的5.1版本注释
The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
作者信息
Smith Christopher D, Shu Shengqiang, Mungall Christopher J, Karpen Gary H
机构信息
Department of Biology, San Francisco State University, San Francisco, CA 94132, USA.
出版信息
Science. 2007 Jun 15;316(5831):1586-91. doi: 10.1126/science.1139815.
Abstract
The repetitive DNA that constitutes most of the heterochromatic regions of metazoan genomes has hindered the comprehensive analysis of gene content and other functions. We have generated a detailed computational and manual annotation of 24 megabases of heterochromatic sequence in the Release 5 Drosophila melanogaster genome sequence. The heterochromatin contains a minimum of 230 to 254 protein-coding genes, which are conserved in other Drosophilids and more diverged species, as well as 32 pseudogenes and 13 noncoding RNAs. Improved methods revealed that more than 77% of this heterochromatin sequence, including introns and intergenic regions, is composed of fragmented and nested transposable elements and other repeated DNAs. Drosophila heterochromatin contains "islands" of highly conserved genes embedded in these "oceans" of complex repeats, which may require special expression and splicing mechanisms.
摘要
构成后生动物基因组大部分异染色质区域的重复DNA阻碍了对基因含量和其他功能的全面分析。我们对黑腹果蝇基因组序列第5版中24兆碱基的异染色质序列进行了详细的计算和人工注释。异染色质至少包含230至254个蛋白质编码基因，这些基因在其他果蝇类和分化程度更高的物种中保守，还包含32个假基因和13个非编码RNA。改进的方法表明，该异染色质序列中超过77%，包括内含子和基因间区域，由片段化和嵌套的转座元件及其他重复DNA组成。果蝇异染色质在这些复杂重复序列的“海洋”中包含高度保守基因的“岛屿”，这可能需要特殊的表达和剪接机制。
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