母马年龄和恢复方法对用于体外成熟程序的马卵泡卵母细胞回收率的影响。

Effect of mare's age and recovery methods on the recovery rate of equine follicular oocytes for IVM procedures.

作者信息

Erice I, Gil L, Josa A, Echegaray A, Martinez F, Espinosa E

机构信息

Department of Animal Pathology, Veterinary Faculty, University of Zaragoza, Spain.

出版信息

Theriogenology. 1998 Mar;49(4):735-41. doi: 10.1016/S0093-691X(98)00022-3.

DOI:10.1016/S0093-691X(98)00022-3

PMID:10732081

Abstract

Mares (n = 39) were classified according to age as young (less than 1.5 yr, n = 17) or old (more than 1.5 yr, n = 22) and sacrificed. Ovaries were measured and weighed, and the number of follicles and CL were counted. Follicle size and distribution were recorded (external: > 20 mm, < 20 mm; internal: > 5 mm, < 5 mm). External follicles were aspirated while internal follicles were sliced. The number and Type of oocytes recovered using each method were recorded. Oocyte recovery rates (oocytes/ovary) resulted in a mean of 0.92 oocytes by aspiration and 1.36 oocytes by additional slicing. The mean numbers of available follicles (8.11 and 5.02) oocytes recovered (4.94 and 3.02) and oocytes selected per ovary (3.29 and 2.32) were not significantly different in the young or old mares, respectively.

摘要

将39匹母马按年龄分为年轻组（小于1.5岁，n = 17）和老年组（大于1.5岁，n = 22），然后对其实施安乐死。测量并称重卵巢，统计卵泡和黄体的数量。记录卵泡大小及分布情况（外部：> 20 mm，< 20 mm；内部：> 5 mm，< 5 mm）。抽吸外部卵泡，将内部卵泡切片。记录每种方法回收的卵母细胞数量及类型。卵母细胞回收率（卵母细胞/卵巢）经抽吸平均为0.92个卵母细胞，经额外切片平均为1.36个卵母细胞。年轻母马和老年母马每组卵巢中可利用卵泡的平均数量（分别为8.11和5.02）、回收的卵母细胞数量（分别为4.94和3.02）以及每个卵巢选择的卵母细胞数量（分别为3.29和2.32）均无显著差异。