McDougall K, Hay M A, Goodrowe K L, Gartley C J, King W A
Department of Biomedical Sciences, University of Guelph, Ontario, Canada.
J Reprod Fertil Suppl. 1997;51:25-31.
Ovaries were collected from prepubertal (< 6 months of age, n = 4 ovaries), peripubertal (6 to 10 months of age, n = 12 ovaries) and mature (> 10 months, n = 12 ovaries) bitches after routine ovariohysterectomies and fixed in formalin. Ovaries were bisected, embedded in paraffin wax and 20 serial sections were made at intervals of 10 microns. Sections were stained with haematoxylin and eosin to examine follicles and oocytes in a cross-section of cortex of known size. Counts were made on all sections, resulting in examination of the entire cortical area present in the sections. Oocytes were counted and classified as nucleate or anucleate. Follicles were counted and classified as large (> 100 microns in diameter) or small (< 100 microns in diameter), containing one oocyte (monovular), more than one oocyte (polyovular) or no oocytes (anovular). It was concluded that at first oestrus there was an increase (P < 0.05) per section in number of total oocytes and small monovular follicles and a significant increase (P < 0.05) in the number of nucleated oocytes in monovular follicles, suggesting that oogenesis or folliculogenesis is still occurring at this age. At pre- and peripubertal ages polyovular follicles were found which persist into maturity. There was no difference in numbers of anovular follicles and total number of polyovular follicles among different age groups.
在常规卵巢子宫切除术后，从青春期前（<6月龄，n = 4个卵巢）、青春期前后（6至10月龄，n = 12个卵巢）和成熟（>10月龄，n = 12个卵巢）的母犬收集卵巢，并固定于福尔马林中。将卵巢切成两半，包埋于石蜡中，每隔10微米制作20个连续切片。切片用苏木精和伊红染色，以检查已知大小皮质横切面中的卵泡和卵母细胞。对所有切片进行计数，从而检查切片中存在的整个皮质区域。对卵母细胞进行计数并分类为有核或无核。对卵泡进行计数并分类为大卵泡（直径>100微米）或小卵泡（直径<100微米），包含一个卵母细胞（单卵卵泡）、一个以上卵母细胞（多卵卵泡）或无卵母细胞（无卵卵泡）。得出的结论是，在第一次发情时，每切片的总卵母细胞和小单卵卵泡数量增加（P<0.05），单卵卵泡中有核卵母细胞数量显著增加（P<0.05），这表明在这个年龄仍在发生卵子发生或卵泡发生。在青春期前和青春期前后的年龄段发现了多卵卵泡，这些卵泡持续到成熟。不同年龄组之间无卵卵泡数量和多卵卵泡总数没有差异。
