Vlásková H, Krásný L, Fucík V, Jonák J
Department of Protein Biosynthesis, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague.
Folia Biol (Praha). 1998;44(5):163-72.
The Bacillus stearothermophilus pyrAb gene, encoding the large subunit of carbamoylphosphate synthetase, was isolated and characterized. The DNA sequence is a 3195-nucleotide long reading frame coding for a polypeptide of 1064 amino acids and deduced Mr approximately 116,160 Da. The pyrAb gene is part of the B. stearothermophilus pyrimidine biosynthesis operon pyr. The 5' end of thepyrAb gene overlaps with the 3' end of the pyrAa gene coding for the small subunit of carbamoylphosphate synthetase, while the 3' end of the pyrAb gene is overlapped with the 5' end of the pyrD gene, which by analogy with the B. subtilis genomic organization, codes for dihydroorotate dehydrogenase. The deduced amino acid sequence of the large subunit of B. stearothermophilus carbamoylphosphate synthetase was compared with the known sequences of the large subunit of carbamoylphosphate synthetase enzymes from other organisms via the NCBI database. Extremely high (98%) identity in amino acid sequence with the large subunit of carbamoylphosphate synthetase from Bacillus caldolyticus was detected.
分离并鉴定了嗜热脂肪芽孢杆菌的pyrAb基因，该基因编码氨甲酰磷酸合成酶的大亚基。DNA序列是一个3195个核苷酸的长读框，编码一个1064个氨基酸的多肽，推导的分子量约为116,160道尔顿。pyrAb基因是嗜热脂肪芽孢杆菌嘧啶生物合成操纵子pyr的一部分。pyrAb基因的5'端与编码氨甲酰磷酸合成酶小亚基的pyrAa基因的3'端重叠，而pyrAb基因的3'端与pyrD基因的5'端重叠，根据枯草芽孢杆菌的基因组组织类推，pyrD基因编码二氢乳清酸脱氢酶。通过NCBI数据库，将嗜热脂肪芽孢杆菌氨甲酰磷酸合成酶大亚基的推导氨基酸序列与来自其他生物体的氨甲酰磷酸合成酶大亚基的已知序列进行了比较。检测到与嗜热解淀粉芽孢杆菌氨甲酰磷酸合成酶大亚基的氨基酸序列具有极高（98%）的同一性。
