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[鸡毒支原体基因组中包含II型DNA拓扑异构酶(topIIB)ATP结合亚基基因的片段核苷酸序列的克隆与分析]

[Cloning and analysis of the nucleotide sequence of the segment in the Mycoplasma gallisepticum genome containing the gene for the ATP-binding subunit of DNA topoisomerase type II (topIIB)].

作者信息

Skamrov A V, Feoktistova E S, Bibilashvili R Sh

出版信息

Mol Biol (Mosk). 1995 Mar-Apr;29(2):308-16.

PMID:7783737
Abstract

M. gallisepticum genome fragment carrying complete coding sequence for ATP-binding subunit of topoisomerase II (topIIB), partial coding sequence for N-terminal part of A-subunit of topoisomerase II and region upstream of topIIB gene (open reading frame encoding 99 amino acids) was sequenced. The nucleotide sequence of topIIB has significant homology with previously reported gyrase genes and parE gene of E. coli. No protein sequence significantly similar to the open reading frame upstream from the gene topIIB was found in GeneBank data.

摘要

对携带拓扑异构酶II(topIIB)ATP结合亚基完整编码序列、拓扑异构酶II A亚基N端部分编码序列以及topIIB基因上游区域(编码99个氨基酸的开放阅读框)的鸡毒支原体基因组片段进行了测序。topIIB的核苷酸序列与先前报道的大肠杆菌促旋酶基因和parE基因具有显著同源性。在基因库数据中未发现与topIIB基因上游开放阅读框显著相似的蛋白质序列。

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