Petrovic D, Zorc M, Zorc-Pleskovic R, Vraspir-Porenta O
Institute of Histology and Embryology, Medical Faculty, University of Ljubljana, Slovenia.
Folia Biol (Praha). 1999;45(2):63-6.
Mast cells play a certain role in inflammation and immunological reactions. Cardiac mast cells, shown by sodium sulfate-alcian blue staining, were evaluated in endomyocardial biopsy specimens in patients with unexplained congestive heart failure. The results of histopathological analysis were consistent with active myocarditis according to the Dallas criteria in 10 patients (15%), borderline myocarditis in 9 (13.8%), and dilated cardiomyopathy in 25 patients (38.5%); these results were compared with a control group of 10 traffic accident victims. The highest numerical areal density of mast cells was found in active myocarditis (3.92 counts/mm2, SD = 1.84), followed by borderline myocarditis (2.76 counts/mm2, SD = 1.66), dilated cardiomyopathy (1.56 counts/mm2, SD = 0.45) and control group (0.77 counts/mm2, SD = 0.19). Degranulation involved 27% (SD = 3.6) of mast cells in active myocarditis, 18% (SD = 4.5) of mast cells in borderline myocarditis, 10.8% (SD = 3.12) of mast cells in dilated cardiomyopathy and 4% (SD = 2.0) of mast cells from autopsy tissue. The differences among the four groups were statistically significant (P <0.001). The increased number of mast cells and the higher degree of their degranulation in myocarditis compared to dilated cardiomyopathy and to control group indicate that they were activated. The mast cells could be involved in modulation of fibrous response, since they tended to be associated with areas of fibrosis. Likewise, numerical areal density and degree of degranulation of mast cells could also be used as additional diagnostic criteria for acute myocarditis, since a higher numerical areal density and degree of degranulation were present in myocarditis vs. dilated cardiomyopathy and control group.
肥大细胞在炎症和免疫反应中发挥着一定作用。通过硫酸铝-阿尔辛蓝染色显示的心脏肥大细胞，在不明原因的充血性心力衰竭患者的心内膜心肌活检标本中进行了评估。根据达拉斯标准，组织病理学分析结果显示，10例患者（15%）符合活动性心肌炎，9例（13.8%）为临界性心肌炎，25例患者（38.5%）为扩张型心肌病；这些结果与10名交通事故受害者的对照组进行了比较。肥大细胞的最高面密度数值出现在活动性心肌炎中（3.92个/mm²，标准差 = 1.84），其次是临界性心肌炎（2.76个/mm²，标准差 = 1.66）、扩张型心肌病（1.56个/mm²，标准差 = 0.45）和对照组（0.77个/mm²，标准差 = 0.19）。脱颗粒现象在活动性心肌炎中涉及27%（标准差 = 3.6）的肥大细胞，临界性心肌炎中为18%（标准差 = 4.5），扩张型心肌病中为10.8%（标准差 = 3.12），尸检组织中的肥大细胞为4%（标准差 = 2.0）。四组之间的差异具有统计学意义（P <0.001）。与扩张型心肌病和对照组相比，心肌炎中肥大细胞数量增加且脱颗粒程度更高，表明它们被激活。肥大细胞可能参与纤维反应的调节，因为它们往往与纤维化区域相关。同样，肥大细胞的面密度数值和脱颗粒程度也可作为急性心肌炎的额外诊断标准，因为心肌炎中的面密度数值和脱颗粒程度高于扩张型心肌病和对照组。
