Gregory M S, Faunce D E, Duffner L A, Kovacs E J
Department of Cell Biology, Neurobiology, and Anatomy, Burn and Shock Trauma Institute, Loyola University Medical Center, Maywood, Illinois 60513, USA.
J Leukoc Biol. 2000 Mar;67(3):319-26.
The gender difference in normal immune function has been well documented, however, there is only limited information regarding whether such a difference occurs after injury. To investigate this, we examined cell-mediated immune responses in male and female mice given a 15% total body surface area dorsal scald or sham injury. Both delayed-type hypersensitivity (DTH) and splenocyte proliferative responses were significantly suppressed in males at 1 day and in females at 7 and 10 days post burn (P < 0.01). The decreased splenocyte proliferation was found to be macrophage-dependent and suppression of both immune parameters corresponded with elevated interleukin-6 (IL-6) levels. Furthermore, post-burn treatment with an anti-IL-6 antibody partially restored the DTH response in males at 1 day and females at 10 days post injury and completely restored splenocyte proliferation. These data demonstrate a possible mechanism for the gender difference in cell-mediated immune responses after thermal injury.
正常免疫功能中的性别差异已有充分记录，然而，关于损伤后是否存在这种差异的信息却很有限。为了对此进行研究，我们检测了给予15%体表面积背部烫伤或假损伤的雄性和雌性小鼠的细胞介导免疫反应。在烧伤后第1天，雄性小鼠的迟发型超敏反应（DTH）和脾细胞增殖反应均显著受到抑制，而在烧伤后第7天和第10天，雌性小鼠的上述反应受到显著抑制（P < 0.01）。发现脾细胞增殖减少是巨噬细胞依赖性的，并且两种免疫参数的抑制都与白细胞介素-6（IL-6）水平升高相对应。此外，烧伤后用抗IL-6抗体治疗可部分恢复损伤后第1天雄性小鼠和第10天雌性小鼠的DTH反应，并完全恢复脾细胞增殖。这些数据证明了热损伤后细胞介导免疫反应中性别差异的一种可能机制。
