Wang A W, Politch J, Anderson D
Department of Obstetrics, Gynecology and Reproductive Biology, Brigham and Women's Hospital, Harvard Medical School, Boston.
Andrologia. 1994 May-Jun;26(3):167-72. doi: 10.1111/j.1439-0272.1994.tb00783.x.
Recent studies have revealed a high prevalence of leukocytospermia (> 1 x 10(6) white blood cells ml-1 semen) in male infertility patients in the USA and certain European countries, and have implicated white blood cells as a cause of infertility. Since leukocytospermia may often be attributed to male genital-tract infections, its prevalence could vary widely in different populations depending on factors such as sexual practices and the prevalence of sexually transmitted pathogens. In the study described here the incidence of leukocytospermia was determined in a group of 101 male infertility patients and a small reference group of normal fertile men in Beijing, China. Seminal white blood cells (WBC) and WBC sub-populations were enumerated by peroxidase staining and immunohistological assay. Eight out of 101 (7.9%) samples from infertility patients and 0/10 samples from fertile donors were leukocytospermic. The incidence of leukocytospermia in the Chinese infertility patients was considerably lower than the 23% incidence observed in a recent study of infertility patients in the USA using a similar technique. All but one of the patients with leukocytospermia had a poor sperm count and/or poor sperm motility. However, due to the low incidence of leukocytospermia and the small number of patients in this group, a statistically significant association between leukocytospermia and poor semen quality was not attained. The simple peroxidase test correlated well with the more expensive and technically demanding immunohistological assay for detection of white blood cells in semen.
最近的研究表明，在美国和某些欧洲国家的男性不育患者中，白细胞精子症（>1×10⁶个白细胞/毫升精液）的患病率很高，并且认为白细胞是导致不育的一个原因。由于白细胞精子症往往可能归因于男性生殖道感染，其患病率在不同人群中可能因性行为和性传播病原体的流行率等因素而有很大差异。在本文所述的研究中，对中国北京的101名男性不育患者和一小群正常生育男性的白细胞精子症发病率进行了测定。通过过氧化物酶染色和免疫组织学检测对精液中的白细胞（WBC）和白细胞亚群进行计数。101份不育患者样本中有8份（7.9%）以及10份生育男性供体样本中有0份为白细胞精子症。中国不育患者中白细胞精子症的发病率明显低于最近在美国使用类似技术对不育患者进行的一项研究中观察到的23%的发病率。除一名患者外，所有白细胞精子症患者的精子计数和/或精子活力都很差。然而，由于白细胞精子症的发病率较低且该组患者数量较少，未得出白细胞精子症与精液质量差之间具有统计学意义的关联。简单的过氧化物酶检测与检测精液中白细胞的更昂贵且技术要求更高的免疫组织学检测结果相关性良好。
