Schacht C J, Foote R H
Department of Animal Science, Cornell University, Ithaca, NY 14853-4801, USA.
Reprod Nutr Dev. 2000 Jan-Feb;40(1):77-86. doi: 10.1051/rnd:2000121.
Survival of embryos exposed to several concentrations of uterine proteins and changes in tubal morphology in rabbits given low preovulatory doses of progesterone (P4) that had previously not affected ovulation or fertilization, but caused severe embryo mortality, were studied. In experiment 1, 332 morulae were cultured for 24 h in a control medium containing < 0.5 to > 3.0 mg x mL(-1) of Day 3 uterine fluid proteins. There was no difference in blastocyst development nor implantation to Day 12 following transfer of the blastocysts to recipients, except fewer implants developed in the BSA control. In experiment 2 the oviducts and uteri of control and P4-treated does were examined by SEM for 8 days following ovulation. Secretory cells in the oviducts and to a lesser extent in the uteri were stimulated by P4 treatment for 3 to 4 days after ovulation. Morphology of ciliated cells was unaffected. The subtle changes did not fully account for P4-induced embryo mortality in vivo.
研究了暴露于几种浓度子宫蛋白的胚胎的存活率，以及给排卵前低剂量孕酮（P4）的兔子输卵管形态的变化，这些兔子此前并未影响排卵或受精，但导致了严重的胚胎死亡。在实验1中，332个桑椹胚在含有<0.5至>3.0 mg x mL(-1)第3天子宫液蛋白的对照培养基中培养24小时。将囊胚移植到受体后，囊胚发育和到第12天的着床情况没有差异，只是在牛血清白蛋白对照中发育的着床较少。在实验2中，在排卵后8天通过扫描电子显微镜检查对照和P4处理母兔的输卵管和子宫。排卵后3至4天，P4处理刺激了输卵管中的分泌细胞以及子宫中程度较轻的分泌细胞。纤毛细胞的形态未受影响。这些细微变化并不能完全解释P4在体内诱导的胚胎死亡。
