Cline E M, Randall P A, Oliphant G
Fertil Steril. 1977 Jul;28(7):766-71.
This study presents evidence that estradiol, mediated through oviduct fluid, may adversely affect the development of early embryos. Two-cell mouse embryos were cultured in Whitten-Biggers medium with or without 0.2 to 20 microgram/ml of estradiol or progesterone or in mouse oviduct fluid from donors treated with estradiol or estradiol and progesterone. Embryos cultured in fluid from estrogen-dominated donors were significantly less able to develop to morula (65%) or blastocyst (14%) than were those cultured in either Whitten-Biggers medium (91% and 41%) or progesterone-dominated fluid (87% and 36%). Transfer of cultured morulae and blastocysts to uteri of pseudopregnant recipients resulted in 2/3, 4/6, and 0/7 recipients' becoming pregnant for control, progesterone-dominated, and estrogen-dominated, respectively. When estradiol or progesterone was added directly to the control medium, no inhibitory effects were observed at levels higher than those found physiologically in plasma. Protein concentration and osmolalities for representative samples of oviduct fluid were 5.2 mg/ml and 329 mOsM for estrogen-dominated fluid and 1.6 mg/ml and 339 mOsM for progesterone-dominated fluid.
本研究表明，通过输卵管液介导的雌二醇可能对早期胚胎的发育产生不利影响。将二细胞期小鼠胚胎培养于含或不含0.2至20微克/毫升雌二醇或孕酮的惠滕-比格斯培养基中，或培养于用雌二醇或雌二醇与孕酮处理过的供体的小鼠输卵管液中。与培养于惠滕-比格斯培养基（桑椹胚发育率91%，囊胚发育率41%）或孕酮主导的液体（桑椹胚发育率87%，囊胚发育率36%）中的胚胎相比，培养于雌激素主导的供体的液体中的胚胎发育至桑椹胚（65%）或囊胚（14%）的能力明显较低。将培养的桑椹胚和囊胚移植到假孕受体的子宫中，对照组、孕酮主导组和雌激素主导组受体的妊娠率分别为2/3、4/6和0/7。当将雌二醇或孕酮直接添加到对照培养基中时，在高于血浆中生理水平的情况下未观察到抑制作用。雌激素主导的液体的输卵管液代表性样本的蛋白质浓度和渗透压分别为5.2毫克/毫升和329毫渗摩尔，孕酮主导的液体为1.6毫克/毫升和339毫渗摩尔。
