Gill H S, Rutherfurd K J, Prasad J, Gopal P K
Milk and Health Research Centre, Institute of Food, Nutrition and Human Health, Massey University, Palmerston North, New Zealand.
Br J Nutr. 2000 Feb;83(2):167-76. doi: 10.1017/s0007114500000210.
Consumption of lactic acid bacteria (LAB) has been suggested to confer a range of health benefits including stimulation of the immune system and increased resistance to malignancy and infectious illness. In the present study, the effects of feeding Lactobacillus rhamnosus (HN001, DR20), Lactobacillus acidophilus (HN017) and Bifidobacterium lactis (HN019, DR10) on in vivo and in vitro indices of natural and acquired immunity in healthy mice were examined. Mice were fed daily with L. rhamnosus, L. acidophilus or B. lactis (10(9) colony forming units) and their immune function was assessed on day 10 or day 28. Supplementation with L. rhamnosus, L. acidophilus or B. lactis resulted in a significant increase in the phagocytic activity of peripheral blood leucocytes and peritoneal macrophages compared with the control mice. The proliferative responses of spleen cells to concanavalin A (a T-cell mitogen) and lipopolysaccharide (a B-cell mitogen) were also significantly enhanced in mice given different LAB. Spleen cells from mice given L. rhamnosus, L. acidophilus or B. lactis also produced significantly higher amounts of interferon-gamma in response to stimulation with concanavalin A than cells from the control mice. LAB feeding had no significant effect on interleukin-4 production by spleen cells or on the percentages of CD4+, CD8+ and CD40+ cells in the blood. The serum antibody responses to orally and systemically administered antigens were also significantly enhanced by supplementation with L. rhamnosus, L. acidophilus or B. lactis. Together, these results suggest that supplementation of the diet with L. rhamnosus (HN001), L. acidophilus (HN017) or B. lactis (HN019) is able to enhance several indices of natural and acquired immunity in healthy mice.
食用乳酸菌（LAB）已被认为具有一系列健康益处，包括刺激免疫系统以及增强对恶性肿瘤和传染病的抵抗力。在本研究中，检测了喂食鼠李糖乳杆菌（HN001、DR20）、嗜酸乳杆菌（HN017）和双歧杆菌（HN019、DR10）对健康小鼠体内和体外天然免疫及获得性免疫指标的影响。小鼠每天喂食鼠李糖乳杆菌、嗜酸乳杆菌或双歧杆菌（10⁹ 菌落形成单位），并在第10天或第28天评估其免疫功能。与对照小鼠相比，补充鼠李糖乳杆菌、嗜酸乳杆菌或双歧杆菌可使外周血白细胞和腹腔巨噬细胞的吞噬活性显著增加。给予不同乳酸菌的小鼠脾脏细胞对伴刀豆球蛋白A（一种T细胞有丝分裂原）和脂多糖（一种B细胞有丝分裂原）的增殖反应也显著增强。给予鼠李糖乳杆菌、嗜酸乳杆菌或双歧杆菌的小鼠脾脏细胞在伴刀豆球蛋白A刺激下产生的干扰素-γ 量也显著高于对照小鼠的细胞。喂食乳酸菌对脾脏细胞产生白细胞介素-4或血液中CD4⁺、CD8⁺ 和CD40⁺ 细胞的百分比没有显著影响。补充鼠李糖乳杆菌、嗜酸乳杆菌或双歧杆菌也显著增强了对口服和全身给药抗原的血清抗体反应。总之，这些结果表明，在饮食中补充鼠李糖乳杆菌（HN001）、嗜酸乳杆菌（HN017）或双歧杆菌（HN019）能够增强健康小鼠的多种天然免疫和获得性免疫指标。
