IFF Health & Nutrition, Sokeritehtaantie 20, 02460 Kantvik, Finland.
Nutrients. 2023 Aug 11;15(16):3541. doi: 10.3390/nu15163541.
subsp. HN019 is a probiotic with several documented human health benefits. Interest in probiotics has led to the development of new formats that probiotics, including HN019, can be supplemented into. In this study, we looked at common HN019 formats such as frozen culture and freeze-dried powder as well as supplementing it into the following food matrices: yogurts (dairy, soy, and oat based), xanthan gum-based tablets, pulpless orange juice, whey sports drink, and dark chocolate (70% cocoa). In this work, our aim was to investigate whether the food matrix that carried HN019 via simulated human digestion (a dual model system mimicking both upper and lower gastrointestinal digestion) influenced probiotic delivery. To that end, we validated and used a real-time qPCR assay to detect HN019 after simulated digestion. In addition, we also measured the effect on a panel of metabolites. After simulated digestion, we were able to detect HN019 from all the matrices tested, and the observed changes to the metabolite profile were consistent with those expected from the food matrix used. In conclusion, this work suggests that the food matrix supplemented with HN019 did not interfere with delivery to the colon via simulated human digestion.
HN019 亚种是一种具有多种已被证实的人类健康益处的益生菌。人们对益生菌的兴趣促使开发出了新的形式，可以将益生菌（包括 HN019）添加到这些新形式中。在这项研究中，我们研究了常见的 HN019 形式，如冷冻培养物和冷冻干燥粉末，以及将其添加到以下食品基质中：酸奶（乳、大豆和燕麦基）、黄原胶基片剂、无果肉橙汁、乳清运动饮料和黑巧克力（可可含量 70%）。在这项工作中，我们的目的是研究通过模拟人体消化（模拟上消化道和下消化道消化的双模型系统）携带 HN019 的食品基质是否会影响益生菌的输送。为此，我们验证并使用实时 qPCR 测定法来检测模拟消化后的 HN019。此外，我们还测量了对一组代谢物的影响。经过模拟消化，我们能够从所有测试的基质中检测到 HN019，并且观察到代谢物谱的变化与所用食品基质所预期的一致。总之，这项工作表明，添加 HN019 的食品基质不会干扰通过模拟人体消化输送到结肠。
