García F, Garcia F, López I, Bernal C, Hernandez J, García-Valdecasas J, Piédrola G, Maroto C
Service of Microbiology, University Hospital San Cecilio, Granada, Spain.
J Basic Microbiol. 2000;40(1):25-32.
Our study evaluates the analytical performance of two amplification methods in the detection of GB Virus C/Hepatitis G Virus, GEN ETI-K HGV RNA (GEN) and the LCx GBV-C Assay (LCx). GB Virus C RNA was detected by at least one test in 58/315 samples (18.41%). Fifty-five samples (17.46%) were positive by the GEN method and 51 samples (16.19%) by the LCx method. The same rate of detection was found for 71 haemodialysis patients and 18 non-A non-E hepatitis. Method based differences in prevalence were observed for patient samples from the general population, 8/106 (7.55%) positive by GEN vs 7/106 (6.60%) by LCx; and HIV infected patients, 26/98 (26.53%) vs 23/98 (23.46%). For chronic type C hepatitis 10/22 (45.5%) were positive by both methods, with two samples discordant. Overall, discordance was observed for ten samples, with seven positive only by the GEN ETI-K HGV RNA, and three positive only by the LCx GBV-C Assay. An additional evaluation of serial samples, from chronic type C hepatitis patients under interferon treatment, revealed three samples which were positive only by the GEN method. Results were 100% concordant for patients under haemodialysis and for non-A non-E hepatitis, 95.9% in the HIV positive group, 90.9% in the chronic type C hepatitis group, and 97.1% in the general population group. Overall, a 97.2% of concordance was found between methods. Both tests have a similar diagnostic performance, though in our opinion, LCx GBV-C Assay better suits the requirements of a clinical microbiology diagnostic laboratory.
我们的研究评估了两种扩增方法在检测庚型肝炎病毒（GB Virus C/Hepatitis G Virus）、GEN ETI-K HGV RNA（GEN）和LCx GBV-C检测法（LCx）中的分析性能。在315份样本中，至少有一项检测方法检测到庚型肝炎病毒RNA的样本有58份（18.41%）。GEN方法检测出55份样本（17.46%）呈阳性，LCx方法检测出51份样本（16.19%）呈阳性。在71例血液透析患者和18例非甲非戊型肝炎患者中，两种方法的检测率相同。在来自普通人群的患者样本中，观察到方法差异导致的患病率不同，GEN方法检测出8/106（7.55%）呈阳性，而LCx方法检测出7/106（6.60%）呈阳性；在HIV感染患者中，GEN方法检测出26/98（26.53%）呈阳性，LCx方法检测出23/98（23.46%）呈阳性。对于慢性丙型肝炎患者，两种方法均检测出10/22（45.5%）呈阳性，有两份样本结果不一致。总体而言，观察到10份样本结果不一致，其中7份仅通过GEN ETI-K HGV RNA检测呈阳性，3份仅通过LCx GBV-C检测法检测呈阳性。对接受干扰素治疗的慢性丙型肝炎患者的系列样本进行的额外评估显示，有3份样本仅通过GEN方法检测呈阳性。血液透析患者和非甲非戊型肝炎患者的检测结果100%一致，HIV阳性组的一致率为95.9%，慢性丙型肝炎组为90.9%，普通人群组为97.1%。总体而言，两种方法之间的一致率为97.2%。两种检测方法具有相似的诊断性能，不过在我们看来，LCx GBV-C检测法更符合临床微生物诊断实验室的要求。
