Tabrizi S N, McCurrach F E, Drewe R H, Borg A J, Garland S M, Taylor H R
Department of Microbiology, Royal Women's Hospital, Carlton, Victoria, Australia.
Aust N Z J Ophthalmol. 1997 Aug;25(3):211-5. doi: 10.1111/j.1442-9071.1997.tb01394.x.
To investigate the role of human papillomavirus (HPV) types, 6, 11, 16 and 18 in corneal and conjunctival carcinoma, we examined 88 dysplastic corneal and conjunctival specimens and 66 controls that had been formalin-fixed and paraffin-embedded.
Sections were graded for histological abnormality by light microscopy and the presence of HPV DNA was determined by polymerase chain reaction using LI consensus primers.
Human papillomavirus DNA was detected in 34 (39%) dysplasias and in five (7.5%) controls. Of dysplasias that were HPV-positive, 20 (59%) contained either types 16 or 18, 13 (38%) contained only types 6/11, while combinations of HPV types were present in 11 (32%). A histological correlation was found with HPV positivity (all genotypes) and unusually large ('epithelioid') dysplastic cells.
The present study demonstrates a lower incidence of HPV in corneal and conjunctival carcinoma than previously reported, but shows an unexpectedly high incidence of HPV 6/11 in conjunctival carcinomas.
为了研究人乳头瘤病毒（HPV）6、11、16和18型在角膜和结膜癌中的作用，我们检查了88份经福尔马林固定和石蜡包埋的发育异常角膜和结膜标本以及66份对照标本。
通过光学显微镜对切片的组织学异常进行分级，并使用LI共有引物通过聚合酶链反应确定HPV DNA的存在。
在34份（39%）发育异常标本和5份（7.5%）对照标本中检测到了人乳头瘤病毒DNA。在HPV阳性的发育异常标本中，20份（59%）含有16型或18型，13份（38%）仅含有6/11型，11份（32%）存在HPV多种类型的组合。发现HPV阳性（所有基因型）与异常大的（“上皮样”）发育异常细胞之间存在组织学相关性。
本研究表明角膜和结膜癌中HPV的发生率低于先前报道，但结膜癌中HPV 6/11的发生率意外地高。
