Landers P, Kerr K G, Rowbotham T J, Tipper J L, Keig P M, Ingham E, Denton M
Department of Microbiology, University of Leeds, UK.
Eur J Clin Microbiol Infect Dis. 2000 Feb;19(2):121-3. doi: 10.1007/s100960050442.
The aim of this study was to investigate whether Burkholderia cepacia is capable of survival and growth within the free-living amoeba Acanthamoeba polyphaga using a differential immunofluorescence assay of bacterial-amoebal cocultures and viable counts of bacteria determined after amoebal lysis. The numbers of intra-amoebal bacteria and the numbers of infected amoebae increased over time; although, when heat-killed bacteria were used, no intracellular bacteria were observed. These findings should be taken into account in future studies of environmental reservoirs of Burkholderia cepacia.
本研究的目的是使用细菌-变形虫共培养物的差异免疫荧光测定法以及在变形虫裂解后测定的细菌活菌计数，来研究洋葱伯克霍尔德菌是否能够在自由生活的变形虫多噬棘阿米巴中存活和生长。随着时间的推移，变形虫内细菌的数量和被感染变形虫的数量都增加了；然而，当使用热灭活细菌时，未观察到细胞内细菌。在未来关于洋葱伯克霍尔德菌环境储存库的研究中应考虑这些发现。
