Joyanes P, Pascual A, Martínez-Martínez L, Hevia A, Perea E J
Department of Microbiology, School of Medicine, Seville, Spain.
Eur J Clin Microbiol Infect Dis. 2000 Feb;19(2):124-7. doi: 10.1007/s100960050443.
The in vitro adherence of ten strains of Enterococcus faecalis and ten strains of Enterococcus faecium to siliconized latex urinary catheters and to silicone elastomer was evaluated. Bacterial suspensions (2.5x10(5) cfu/ml) in tryptic soy broth containing 0.5 cm segments from each type of catheter were incubated at 37 degrees C. At specified intervals, the segments were washed to remove nonadherent bacteria and sonicated for 1 min, and colony-forming units were quantified. Bacterial adherence occurred rapidly, reaching maximal peaks after 24 h of incubation. Enterococcus faecium adherence to both biomaterials was significantly lower than that of Enterococcus faecalis. No differences were observed between the two elastomers. Bacterial adherence was not related to bacterial surface hydrophobicity, hemolysin or gelatinase production.
对10株粪肠球菌和10株屎肠球菌在硅化乳胶导尿管和硅橡胶上的体外黏附情况进行了评估。将含有每种类型导尿管0.5厘米片段的胰蛋白胨大豆肉汤中的细菌悬液（2.5×10⁵ cfu/ml）在37℃下孵育。在特定时间间隔，将片段冲洗以去除未黏附的细菌并超声处理1分钟，然后对菌落形成单位进行定量。细菌黏附迅速发生，孵育24小时后达到最大峰值。屎肠球菌对两种生物材料的黏附明显低于粪肠球菌。两种弹性体之间未观察到差异。细菌黏附与细菌表面疏水性、溶血素或明胶酶的产生无关。
