Gabriel M M, Sawant A D, Simmons R B, Ahearn D G
Biology Department, Georgia State University, Atlanta 30302-4010.
Curr Microbiol. 1995 Jan;30(1):17-22. doi: 10.1007/BF00294518.
Strains of Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa, Enterococcus faecalis, and Klebsiella pneumoniae, mostly from complicated urinary tract infections, showed reduced adherence to silver-treated silicone or latex catheters as compared with latex or silicone catheters. The relative degrees of cell adherence to catheters at 2 h or 18 h, as indicated by radiolabeled cell assays, were in general agreement with growth rate-reduction assays and scanning-electron-microscopy data. For strains of E. coli, the correlation between cell hydrophobicity and degree of adherence to catheters was not significant. Antibiotic resistance (tetracycline, sulfathiazine, neomycin, kanamycin) and silver resistance were not associated. The radiolabel adherence procedure provided a quantitative method for evaluating the relative antimicrobial efficacy of silver-treated catheters.
大肠杆菌、奇异变形杆菌、铜绿假单胞菌、粪肠球菌和肺炎克雷伯菌的菌株大多来自复杂性尿路感染，与乳胶或硅胶导管相比，它们对经银处理的硅胶或乳胶导管的黏附性降低。放射性标记细胞试验显示，在2小时或18小时时，细胞对导管的相对黏附程度总体上与生长速率降低试验和扫描电子显微镜数据一致。对于大肠杆菌菌株，细胞疏水性与对导管的黏附程度之间的相关性不显著。抗生素耐药性（四环素、磺胺噻唑、新霉素、卡那霉素）与银耐药性无关。放射性标记黏附程序提供了一种评估经银处理导管相对抗菌效果的定量方法。
