Emili A Q, Cagney G
Department of Genetics and Medicine, University of Washington, Seattle, WA 98195, USA.
Nat Biotechnol. 2000 Apr;18(4):393-7. doi: 10.1038/74442.
The array format for analyzing peptide and protein function offers an attractive experimental alternative to traditional library screens. Powerful new approaches have recently been described, ranging from synthetic peptide arrays to whole proteins expressed in living cells. Comprehensive sets of purified peptides and proteins permit high-throughput screening for discrete biochemical properties, whereas formats involving living cells facilitate large-scale genetic screening for novel biological activities. In the past year, three major genome-scale studies using yeast as a model organism have investigated different aspects of protein function, including biochemical activities, gene disruption phenotypes, and protein-protein interactions. Such studies show that protein arrays can be used to examine in parallel the functions of thousands of proteins previously known only by their DNA sequence.
用于分析肽和蛋白质功能的阵列形式为传统文库筛选提供了一种有吸引力的实验替代方法。最近已经描述了一些强大的新方法，从合成肽阵列到活细胞中表达的完整蛋白质。纯化肽和蛋白质的综合集合允许对离散的生化特性进行高通量筛选，而涉及活细胞的形式则有助于对新型生物活性进行大规模遗传筛选。在过去的一年里，三项以酵母为模式生物的重大基因组规模研究调查了蛋白质功能的不同方面，包括生化活性、基因破坏表型和蛋白质-蛋白质相互作用。这些研究表明，蛋白质阵列可用于并行检查数千种以前仅通过其DNA序列为人所知的蛋白质的功能。
