Mohankumar M N, Venkatachalam P, Prabhu B K, Jeevanram R K
Health and Safety Division, SHINE Group, Indira Gandhi Centre for Atomic Research, Kalpakkam-603 102, India.
Mutat Res. 2000 Feb 14;447(2):199-207. doi: 10.1016/s0027-5107(99)00207-9.
In an attempt to understand and ascertain the stimulatory effects of low-dose ionising radiation, a study was conducted to compare the changes in the UV-induced repair capacity of human blood cells exposed to low conditioning doses of ionising radiation under in vivo and in vitro conditions. A significant increase in the rate of UV induced Unscheduled DNA synthesis (UDS) in lymphocytes pre-exposed to low doses of ionising radiation was observed both under in vitro and in vivo conditions. There was also a significant correlation between the adapting dose and net UDS in lymphocytes of radiation workers implying that the triggering action of the adaptation process is dose dependent. However, on comparing the extent of UV-induced UDS of the in vivo and in vitro exposures, significantly higher rates of UDS were observed in the lymphocytes of radiation workers when compared to a corresponding in vitro adapting dose. We postulate that the response in vivo is much more pronounced due to cell repopulating events and extra cellular secretory factors like hormones etc,.
为了理解和确定低剂量电离辐射的刺激作用，开展了一项研究，以比较在体内和体外条件下，暴露于低预处理剂量电离辐射的人体血细胞紫外线诱导修复能力的变化。在体外和体内条件下，均观察到预先暴露于低剂量电离辐射的淋巴细胞中紫外线诱导的非程序性DNA合成（UDS）速率显著增加。辐射工作人员淋巴细胞的适应剂量与净UDS之间也存在显著相关性，这意味着适应过程的触发作用是剂量依赖性的。然而，在比较体内和体外暴露的紫外线诱导UDS程度时，与相应的体外适应剂量相比，辐射工作人员淋巴细胞中的UDS速率显著更高。我们推测，由于细胞再生事件和激素等细胞外分泌因子，体内反应更为明显。
