Spitkovskiĭ D M, Ermakov A V, Gorin A I, Pospekhova N I, Prokhorov A Iu
Vestn Ross Akad Med Nauk. 1993(4):25-9.
The dependence of UV-induced unscheduled DNA synthesis (UDS) in the unstimulated lymphocytes of human peripheral blood on the ionic strength (mu) of the culture medium has been shown. In the level of mu lower or higher than the physiological (mu ph) one, UDS significantly decreases. The effect of modification of mu due to the changes of ionic strength is absent in the lymphocytes of the classic form of xeroderma pigmentosum. The phenomenon may become useful in the development of a new test for revealing cells with a genetically or physiologically changed system of UV-induced DNA repair. The mechanisms of investigated phenomenon, particularly their dependence on the structure of chromatin, as well as the influence of ionic strength on binding of the repair enzymes with DNA are discussed.
已经表明，人外周血未受刺激淋巴细胞中紫外线诱导的非定标性DNA合成（UDS）对培养基离子强度（μ）具有依赖性。在低于或高于生理水平（μph）的μ水平下，UDS显著降低。经典型着色性干皮病患者的淋巴细胞不存在因离子强度变化而导致的μ改变效应。该现象可能有助于开发一种新的检测方法，以揭示具有紫外线诱导DNA修复系统发生遗传或生理改变的细胞。本文讨论了所研究现象的机制，特别是它们对染色质结构的依赖性，以及离子强度对修复酶与DNA结合的影响。
