Jagetia G C, Adiga S K
Department of Radiobiology, Kasturba Medical College, 3rd Floor, Manipal, Karnataka 576 119, India.
Mutat Res. 2000 Mar 14;448(1):57-68. doi: 10.1016/s0027-5107(99)00240-7.
Effect of 20 nM vindesine sulphate (VDS) treatment was studied on cell survival, growth kinetics and micronuclei induction in V79 cells exposed to 0-300 cGy of gamma-radiation at 16, 22 and 28 h post-irradiation. Treatment of V79 cells with VDS before exposure to different doses of gamma radiation resulted in a significant decline in cell survival and growth kinetic when compared with the concurrent PBS+irradiation group. The decline in cell survival and growth kinetics was dose related. Similarly, the cell proliferation indices also declined in a dose dependent manner in both PBS+irradiation and VDS+irradiation groups and this decline was higher in VDS+irradiation group in comparison with the PBS+irradiation group. In contrast, the frequency of micronuclei increased in a dose related manner in both PBS+irradiation and VDS+irradiation groups. However, the frequency of micronuclei was significantly greater in the VDS+irradiation group when compared to the PBS+irradiation group at all the post-irradiation time periods studied and the dose response for both groups was linear for all the scoring time periods. The biological response was determined by plotting surviving fraction and micronuclei frequencies on X- and Y-axes, respectively. The plot between surviving fraction and micronuclei induction showed a close correlation. The surviving fraction of V79 cells reduced with the increasing frequency of micronuclei in both groups and the relationship between micronuclei induction and cell survival could be fitted on a linear quadratic model.
研究了在照射后16、22和28小时，用20 nM硫酸长春地辛（VDS）处理对暴露于0 - 300 cGyγ射线的V79细胞的细胞存活、生长动力学和微核诱导的影响。与同时进行的PBS +照射组相比，在暴露于不同剂量γ射线之前用VDS处理V79细胞导致细胞存活和生长动力学显著下降。细胞存活和生长动力学的下降与剂量相关。同样，在PBS +照射组和VDS +照射组中，细胞增殖指数也呈剂量依赖性下降，并且VDS +照射组的这种下降比PBS +照射组更高。相反，在PBS +照射组和VDS +照射组中，微核频率均呈剂量相关增加。然而，在所有研究的照射后时间段，VDS +照射组的微核频率均显著高于PBS +照射组，并且两组在所有评分时间段的剂量反应均为线性。通过分别在X轴和Y轴上绘制存活分数和微核频率来确定生物学反应。存活分数与微核诱导之间的关系图显示出密切的相关性。两组中V79细胞的存活分数均随着微核频率的增加而降低，微核诱导与细胞存活之间的关系可以用线性二次模型拟合。
