Jagetia G C, Adiga S K
Department of Radiobiology, Kasturba Medical College, Manipal-576 119, Karnataka, India.
Mutat Res. 1998 Oct 12;421(1):55-63. doi: 10.1016/s0027-5107(98)00154-7.
Effect of different concentrations (0, 1, 5, 10, 20 and 50 nM) of vindesine sulphate was studied on clonogenicity and micronucleus (MN) formation in V79 (Chinese hamster lung fibroblasts) cells. Exposure of V79 cells to vindesine for 6 h resulted in a concentration dependent decline in cell survival. The frequency of micronuclei (MN) increased in a concentration dependent manner at 16, 22 and 28 h post-exposure. The frequency of MN increased significantly after 5 to 50 nM drug exposure at 16 and 22 h post-treatment, while increasing post-exposure time to 28 h resulted in a significant increase in MN frequency at all exposure doses of vindesine. The statistical evaluation of concurrent concentrations at various time periods showed a non-significant difference in MN frequency among various post-exposure time periods, except 16 h and 28 h for 50 nM, where a significant decline in the MN frequency was observed at 28 h compared to 16 h post-exposure. The cell proliferation indices showed a concentration dependent decline in the frequency of binucleate cells and this decline was linear quadratic. The increasing drug concentration resulted in a concentration dependent decline in cell survival. While the frequency of micronuclei increased the cell survival decreased and the relationship between cell survival and micronucleus induction was linear quadratic.
研究了不同浓度（0、1、5、10、20和50 nM）硫酸长春地辛对V79（中国仓鼠肺成纤维细胞）细胞克隆形成能力和微核（MN）形成的影响。将V79细胞暴露于长春地辛6小时导致细胞存活率呈浓度依赖性下降。在暴露后16、22和28小时，微核（MN）频率呈浓度依赖性增加。在处理后16和22小时，5至50 nM药物暴露后MN频率显著增加，而将暴露后时间延长至28小时导致长春地辛所有暴露剂量下MN频率均显著增加。对不同时间段同时存在的浓度进行统计评估显示，除50 nM的16小时和28小时外，不同暴露后时间段的MN频率无显著差异，其中与暴露后16小时相比，28小时时MN频率显著下降。细胞增殖指数显示双核细胞频率呈浓度依赖性下降，且这种下降呈线性二次关系。药物浓度增加导致细胞存活率呈浓度依赖性下降。微核频率增加时细胞存活率下降，细胞存活率与微核诱导之间的关系呈线性二次关系。
