Jagetia G C, Adiga S K
Department of Radiobiology, Kasturba Medical College, India.
Mutat Res. 1997 Jun 9;377(1):105-13. doi: 10.1016/s0027-5107(97)00067-5.
Exposure of V79 cells to different doses of gamma-irradiation resulted in a dose-dependent decline in their survival. The treatment of cells with paclitaxel before irradiation resulted in a further decline in the cell survival. Conversely, the micronuclei frequency increased with the increasing dose of radiation in both irradiated and paclitaxel + irradiated cultures at all the three post-irradiation time periods studied. However, no significant difference among the frequencies of micronuclei was found at 16, 22 and 28 h post-exposure. The trend for cell proliferation was similar to that of cell survival. The cell proliferation declined with increasing dose of radiation. The paclitaxel treatment further reduced cell proliferation compared to the irradiated control group. The dose response for all the parameters for both groups was linear quadratic. The biological response between micronuclei induction and cell survival was also determined. The cell survival and micronuclei formation were inversely related and the correlation between cell survival and micronuclei formation (biological response) was linear quadratic for both irradiated and paclitaxel + irradiated groups.
将V79细胞暴露于不同剂量的γ射线照射下，其存活率呈剂量依赖性下降。在照射前用紫杉醇处理细胞，导致细胞存活率进一步下降。相反，在所有研究的照射后三个时间段，无论是照射组还是紫杉醇 + 照射组，微核频率均随辐射剂量的增加而增加。然而，在暴露后16、22和28小时，微核频率之间未发现显著差异。细胞增殖趋势与细胞存活趋势相似。细胞增殖随辐射剂量增加而下降。与照射对照组相比，紫杉醇处理进一步降低了细胞增殖。两组所有参数的剂量反应均为线性二次关系。还确定了微核诱导与细胞存活之间的生物学反应。细胞存活与微核形成呈负相关，对于照射组和紫杉醇 + 照射组，细胞存活与微核形成（生物学反应）之间的相关性均为线性二次关系。
