17β-雌二醇对人淋巴细胞染色体的遗传毒性作用。

Genotoxic effects of estradiol-17beta on human lymphocyte chromosomes.

作者信息

Ahmad M E, Shadab G G, Hoda A, Afzal M

机构信息

Section of Genetics, Department of Zoology, Aligarh Muslim University, Aligarh, India.

出版信息

Mutat Res. 2000 Mar 3;466(1):109-15. doi: 10.1016/s1383-5718(99)00230-2.

DOI:10.1016/s1383-5718(99)00230-2

PMID:10751732

Abstract

The cytogenetic effect of a hormonal steroid, estradiol-17beta, was assessed in peripheral blood human lymphocyte culture. Sister chromatid exchanges (SCE) and chromosome aberrations (CA) were scored as genetic end points. Significant induction of CA was observed at 25 microg/ml and 50 microg/ml concentrations of estradiol-17beta in the absence of microsomal activation. The drug was effective in all treatments in the presence of rat liver S(9) microsomal fraction (S(9) mix) and exhibited increased frequency of chromosomal aberrations. The drug was effective in increasing the SCE frequency which was found to be maximum at the dose of 50 microg/ml concentration (i.e., 4.34+/-1.22) both with and without metabolic activation. It was found that estradiol-17beta itself and possibly its metabolites are potent mutagens beyond a particular dose in human lymphocytes.

摘要

在人外周血淋巴细胞培养中评估了一种激素甾体——17β-雌二醇的细胞遗传学效应。将姐妹染色单体交换（SCE）和染色体畸变（CA）作为遗传终点进行评分。在无微粒体激活的情况下，当17β-雌二醇浓度为25微克/毫升和50微克/毫升时，观察到CA有显著诱导。在存在大鼠肝脏S(9)微粒体组分（S(9)混合液）的所有处理中，该药物均有效，且染色体畸变频率增加。该药物在增加SCE频率方面有效，发现在50微克/毫升浓度剂量下（即4.34±1.22），无论有无代谢激活，SCE频率均最高。研究发现，17β-雌二醇本身及其可能的代谢产物在超过特定剂量时对人淋巴细胞是强效诱变剂。