Agalarov S C, Sridhar Prasad G, Funke P M, Stout C D, Williamson J R
Department of Molecular Biology and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
Science. 2000 Apr 7;288(5463):107-13. doi: 10.1126/science.288.5463.107.
The crystal structure of a 70-kilodalton ribonucleoprotein complex from the central domain of the Thermus thermophilus 30S ribosomal subunit was solved at 2.6 angstrom resolution. The complex consists of a 104-nucleotide RNA fragment composed of two three-helix junctions that lie at the end of a central helix, and the ribosomal proteins S15, S6, and S18. S15 binds the ribosomal RNA early in the assembly of the 30S ribosomal subunit, stabilizing a conformational reorganization of the two three-helix junctions that creates the RNA fold necessary for subsequent binding of S6 and S18. The structure of the complex demonstrates the central role of S15-induced reorganization of central domain RNA for the subsequent steps of ribosome assembly.
嗜热栖热菌30S核糖体亚基中心结构域的一种70千道尔顿核糖核蛋白复合物的晶体结构在2.6埃分辨率下得到解析。该复合物由一个104个核苷酸的RNA片段组成,该片段由位于中心螺旋末端的两个三螺旋连接点构成,以及核糖体蛋白S15、S6和S18。在30S核糖体亚基组装早期,S15与核糖体RNA结合,稳定了两个三螺旋连接点的构象重排,这种重排产生了后续S6和S18结合所需的RNA折叠。该复合物的结构证明了S15诱导的中心结构域RNA重排在核糖体组装后续步骤中的核心作用。