通过ITS1核糖体分型、UP-PCR指纹图谱和UP-PCR交叉杂交鉴定建筑材料中的木霉菌株。
Identification of Trichoderma strains from building materials by ITS1 ribotyping, UP-PCR fingerprinting and UP-PCR cross hybridization.
作者信息
Lübeck M, Poulsen S K, Lübeck P S, Jensen D F, Thrane U
机构信息
Department of Plant Biology, Plant Pathology Section, Royal Veterinary and Agricultural University, Thorvaldsensvej 40, DK-1871, Frederiksberg C, Denmark.
出版信息
FEMS Microbiol Lett. 2000 Apr 15;185(2):129-34. doi: 10.1111/j.1574-6968.2000.tb09050.x.
To study the role of Trichoderma in sick building syndrome, it is essential to be able to accurately identify species. Forty-four strains of Trichoderma spp. isolated from Danish buildings damaged by water leaks were identified using ITS1 ribotyping and universally primed PCR, UP-PCR. Ribotyping allowed the assignment of the strains into three distinct groups. High similarity of UP-PCR banding profiles of the strains allowed species designation for almost all strains (43 out of 44) when compared with the UP-PCR banding profiles obtained from reference strains of T. atroviride, T. citrinoviride, T. harzianum, T. longibrachiatum and T. viride. However, cross hybridization of UP-PCR products showed that the latter strain had high DNA homology to the ex-type strain of T. hamatum. The combined approach is a convenient way for reliable identification of Trichoderma strains.
为研究木霉在病态建筑综合征中的作用，准确鉴定其物种至关重要。使用ITS1核糖体分型和通用引物PCR（UP-PCR）对从丹麦因漏水受损建筑物中分离出的44株木霉菌株进行了鉴定。核糖体分型可将这些菌株分为三个不同的组。与从深绿木霉、黄绿木霉、哈茨木霉、长枝木霉和绿色木霉参考菌株获得的UP-PCR条带图谱相比，这些菌株的UP-PCR条带图谱高度相似，几乎所有菌株（44株中的43株）都能据此指定物种。然而，UP-PCR产物的交叉杂交表明，后一种菌株与哈氏木霉的模式菌株具有高度的DNA同源性。这种联合方法是可靠鉴定木霉菌株的便捷途径。
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