使用液相色谱串联质谱法（LC/MS/MS）分析猪肾和黑麦粉中的赭曲霉毒素A。

Analysis of ochratoxin A in pig kidney and rye flour using liquid chromatography tandem mass spectrometry (LC/MS/MS).

作者信息

Jørgensen K, Vahl M

机构信息

Institute of Food Research and Nutrition, Danish Veterinary and Food Administration, Soborg, Denmark.

出版信息

Food Addit Contam. 1999 Nov;16(11):451-6. doi: 10.1080/026520399283731.

DOI:10.1080/026520399283731

PMID:10755136

Abstract

A liquid chromatography electrospray tandem mass spectrometric (LC/MS/MS) method is described for analysis and confirmation of ochratoxin A in pig kidney and rye flour using derivatization of ochratoxin A to the methyl ester. Ochratoxin A methyl(d3)ester is used as internal standard. The method works well, the detection limit is 0.02 microgram/kg and the repeatability (coefficient of variation) is between 6% and 16% in the contamination range 0.5 to 8 micrograms/kg.

摘要

描述了一种液相色谱-电喷雾串联质谱（LC/MS/MS）方法，该方法通过将赭曲霉毒素A衍生化为甲酯来分析和确证猪肾和黑麦粉中的赭曲霉毒素A。使用赭曲霉毒素A甲基（d3）酯作为内标。该方法效果良好，检测限为0.02微克/千克，在0.5至8微克/千克的污染范围内，重复性（变异系数）在6%至16%之间。