人嗅鞘细胞移植可引发脱髓鞘大鼠脊髓的髓鞘再生。
Transplantation of human olfactory ensheathing cells elicits remyelination of demyelinated rat spinal cord.
作者信息
Kato T, Honmou O, Uede T, Hashi K, Kocsis J D
机构信息
Department of Neurosurgery, Sapporo Medical University School of Medicine, Sapporo, Japan.
出版信息
Glia. 2000 May;30(3):209-18. doi: 10.1002/(sici)1098-1136(200005)30:3<209::aid-glia1>3.0.co;2-8.
Abstract
Human olfactory ensheathing cells (OECs) were prepared from adult human olfactory nerves, which were removed during surgery for frontal base tumors, and were transplanted into the demyelinated spinal cord of immunosuppressed adult rats. Extensive remyelination was observed in the lesion site: In situ hybridization using a human DNA probe (COT-1) indicated a similar number of COT-1-positive cells and OEC nuclei within the repaired lesion. The myelination was of a peripheral type with large nuclei and cytoplasmic regions surrounding the axons, characteristic of Schwann cell and OEC remyelination. These results provide evidence that adult human OECs are able to produce Schwann cell-like myelin sheaths around demyelinated axons in the adult mammalian CNS in vivo.
摘要
人嗅鞘细胞（OECs）取自成人嗅神经，这些嗅神经是在额叶底部肿瘤手术过程中被切除的，然后被移植到免疫抑制的成年大鼠的脱髓鞘脊髓中。在损伤部位观察到广泛的髓鞘再生：使用人类DNA探针（COT-1）进行的原位杂交表明，修复损伤部位内COT-1阳性细胞和OEC细胞核的数量相似。髓鞘形成属于外周型，有大细胞核和围绕轴突的细胞质区域，这是雪旺细胞和OEC髓鞘再生的特征。这些结果证明，成人OECs能够在成年哺乳动物中枢神经系统的体内脱髓鞘轴突周围产生类似雪旺细胞的髓鞘。
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本文引用的文献
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Transplantation of olfactory ensheathing cells or Schwann cells restores rapid and secure conduction across the transected spinal cord.嗅鞘细胞或施万细胞的移植可恢复经横断脊髓的快速且安全的传导。
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Transplanted olfactory ensheathing cells remyelinate and enhance axonal conduction in the demyelinated dorsal columns of the rat spinal cord.移植的嗅鞘细胞可使大鼠脊髓脱髓鞘的背柱重新髓鞘化并增强轴突传导。
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Low-affinity NGF-receptor and E-N-CAM expression define two types of olfactory nerve ensheathing cells that share a common lineage.低亲和力神经生长因子受体和上皮钙黏蛋白的表达定义了具有共同谱系的两种嗅神经鞘细胞。
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