Wadsworth C, Wadsworth E
Scand J Immunol. 1979 Mar;9(3):255-62. doi: 10.1111/j.1365-3083.1979.tb02729.x.
A simple rapid spot immunoprecipitate assay (SIA) is presented. The antigen--antibody reaction takes place in a field of alternating current; small amounts of specific antisera are used, and the influence of diffusion or electromobility of the antigen is avoided. Determinations of IgG, IgA and IgM in sera from healthy individuals correlated highly significantly with radial immunodiffusion (RID) results. The reaction conditions in SIA, particularly the availability of a sufficiency of specific antibodies, may permit more accurate estimates of monoclonal forms of Ig than in RID, where the combination depends on diffusion of one reactant. The precision of SIA quantitation of IgG and IgA was within +/- 10% and +/- 13% for IgM. Working ranges of the standard curves were from about 25--150 ng for G and A and 75--300 ng for IgM; least squares regression coefficients for four sets of standards on three SIA plates were 0.995 for IgG, 0.969 for IgA, and 0.986 for IgM. IgA at 7.5 ng in a 2.5 mg/l dilution was at the lower limit of demonstrability. Photometric registrations and estimates made by visual inspection compared highly significantly (P less than 0.001), differing by +/- 3%.
本文介绍了一种简单快速的斑点免疫沉淀试验（SIA）。抗原 - 抗体反应在交变电场中进行；使用少量特异性抗血清，避免了抗原扩散或电迁移的影响。对健康个体血清中IgG、IgA和IgM的测定结果与放射免疫扩散（RID）结果高度显著相关。SIA中的反应条件，特别是有足够的特异性抗体，可能比RID更能准确估计Ig的单克隆形式，在RID中，结合取决于一种反应物的扩散。SIA对IgG和IgA定量的精密度在±10%以内，对IgM为±13%。标准曲线的工作范围对于G和A约为25 - 150 ng，对于IgM为75 - 300 ng；在三个SIA板上四组标准品的最小二乘回归系数对于IgG为0.995，对于IgA为0.969，对于IgM为0.986。在2.5 mg/l稀释液中7.5 ng的IgA处于可检测的下限。光度记录和目视检查估计结果高度显著相关（P小于0.001），差异为±3%。
