Cloppet H, Francina A, Coquelin H, Boucaud-Maître Y, Hutinel P, Creyssel R
Clin Chem. 1982 Jan;28(1):180-2.
We evaluated nephelometers from Behring, Hyland, and Beckman for IgG, IgA, and IgM quantitation in sera from patients with monoclonal gammopathies. The intra-batch precision of each instrument for each immunoglobulin class and for different concentrations of the same immunoglobulin was compared to the one obtained with the radial immunodiffusion method. No nephelometer showed a clearly better precision. The correlation with cellulose acetate electrophoresis was good for each of the three nephelometers. The mean value by the radial immunodiffusion method was higher than corresponding determinations by nephelometry.
我们评估了贝林、海兰和贝克曼公司的散射比浊仪，用于定量检测单克隆丙种球蛋白病患者血清中的IgG、IgA和IgM。将每种仪器针对每种免疫球蛋白类别以及相同免疫球蛋白不同浓度的批内精密度与用放射免疫扩散法获得的精密度进行比较。没有一台散射比浊仪显示出明显更好的精密度。三种散射比浊仪与醋酸纤维素电泳的相关性均良好。放射免疫扩散法的平均值高于散射比浊法的相应测定值。
