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SVP的分子克隆:拟南芥中开花转变的负调控因子

Molecular cloning of SVP: a negative regulator of the floral transition in Arabidopsis.

作者信息

Hartmann U, Höhmann S, Nettesheim K, Wisman E, Saedler H, Huijser P

机构信息

Max-Planck-Institut für Züchtungsforschung, Molekulare Pflanzengenetik, Carl-von-Linné-Weg 10, 50829 Cologne, Germany.

出版信息

Plant J. 2000 Feb;21(4):351-60. doi: 10.1046/j.1365-313x.2000.00682.x.

DOI:10.1046/j.1365-313x.2000.00682.x
PMID:10758486
Abstract

Flowering time mutants represent genetic functions in control of the floral transition, an important developmental phase switch in the life cycle of higher plants. Many such mutants have been identified and characterized, particular in Arabidopsis. Here we describe the identification and initial characterization of a new early flowering mutant of Arabidopsis. The corresponding gene, SVP (SHORT VEGETATIVE PHASE), was cloned through transposon tagging and represents a new member of the MADS-box gene family of transcription factors. Analysis of its transcriptional activity revealed the presence of differently sized transcripts that were confined to vegetative tissues and floral primordia and absent from developed flowers and siliques. The function of SVP as a repressor of the floral transition is discussed.

摘要

开花时间突变体代表了控制开花转变的遗传功能,开花转变是高等植物生命周期中一个重要的发育阶段转换。许多这样的突变体已被鉴定和表征,尤其是在拟拟拟拟南芥中。本文我们描述了一个新的拟南芥早花突变体的鉴定和初步表征。通过转座子标签法克隆了相应的基因SVP(SHORT VEGETATIVE PHASE),它代表转录因子MADS-box基因家族的一个新成员。对其转录活性的分析表明,存在大小不同的转录本,这些转录本局限于营养组织和花原基,而在发育成熟的花和角果中不存在。文中还讨论了SVP作为开花转变抑制因子的功能。

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