Yang Xueting, Guo Qi, Liu Shaodan, Wang Duoduo, Zuo Dingding, Niu Tongfei, Wei Dongfeng, Guo Lili, Hou Xiaogai
College of Agriculture / Tree Peony, Henan University of Science and Technology, Luoyang, Henan, 471023, China.
Luoyang International Peony Garden, Luoyang, 471011, China.
BMC Genomics. 2025 Jan 20;26(1):49. doi: 10.1186/s12864-024-11197-y.
Paeonia section Moutan DC. is a significant perennial subshrub, the ornamental value of which heavily depends on the type of flower it possesses. MADS-box transcription factors have a particular impact on the intricate process of floral organ development and differentiation. The release of the whole-genome data from Paeonia ostii now allows us to conduct a thorough investigation of the tree peony MADS-box gene family.
In this study, we identified 110 MADS-box genes in Paeonia ostii that were classified into 5 subgroups. Gene structure, domain and motif analyses revealed the conservation of the structure of these subgroups. Analysis of the cis-acting elements revealed that the 110 PoMADS genes contained different kinds of hormones and stress-related cis-acting elements in their promoter regions. Quantitative real-time PCR analysis was employed to validate the expression patterns of some PoMADS genes related to floral organ development. Genome collinearity analysis with Arabidopsis and grape revealed the conservation of PoMADS genes during evolution. A total of 857 SSRs were identified by analysing the genome sequences of identified genes. We additionally created protein‒protein interaction networks for PoMADS proteins and analysed proteins that could interact among PoMADSs in Arabidopsis thaliana and grape.
These findings offer fundamental insights for understanding the function of the MADS-box gene family, which can aid in the selection and breeding of tree peony varieties with high ornamental value in addition to supporting the understanding of the process of tree peony floral organogenesis.
牡丹组（Paeonia section Moutan DC.）是一种重要的多年生亚灌木，其观赏价值很大程度上取决于其所拥有的花的类型。MADS-box转录因子对花器官发育和分化的复杂过程有特殊影响。紫斑牡丹（Paeonia ostii）全基因组数据的发布，使我们能够对牡丹MADS-box基因家族进行全面研究。
在本研究中，我们在紫斑牡丹中鉴定出110个MADS-box基因，这些基因被分为5个亚组。基因结构、结构域和基序分析揭示了这些亚组结构的保守性。顺式作用元件分析表明，110个牡丹MADS基因在其启动子区域含有不同种类的激素和胁迫相关顺式作用元件。采用定量实时PCR分析来验证一些与花器官发育相关的牡丹MADS基因的表达模式。与拟南芥和葡萄的基因组共线性分析揭示了牡丹MADS基因在进化过程中的保守性。通过分析已鉴定基因的基因组序列，共鉴定出857个简单重复序列（SSRs）。我们还构建了牡丹MADS蛋白的蛋白质-蛋白质相互作用网络，并分析了拟南芥和葡萄中可能在牡丹MADS蛋白之间相互作用的蛋白质。
这些发现为理解MADS-box基因家族的功能提供了基础见解，这不仅有助于理解牡丹花器官发生过程，还能辅助观赏价值高的牡丹品种的选育。
