Watanabe K, Yamashita Y, Nakayama A, Hasegawa Y, Kojima H, Nagasawa T, Mori N
First Department of Pathology, Nagoya University School of Medicine, Nagoya, Japan.
Histopathology. 2000 Apr;36(4):353-61. doi: 10.1046/j.1365-2559.2000.00830.x.
Recent analyses have suggested that Hodgkin's or Reed-Sternberg (HRS) cells in most cases of classic Hodgkin's disease are derived from germinal centre B-cells. However, there is controversy over which B-cell antigens are expressed in HRS cells.
We studied 51 cases of classic Hodgkin's disease to immunohistochemically characterize HRS cells for pan-B-cell markers and specific markers for plasma cells. HRS cells expressed CD20 (L26) in 18 cases (35%), CD19 (B4) in nine (18%) and CD79a (mb-1) in 13 (25%). Furthermore, HRS cells were positive for CD138 (B-B4) in 24 cases (45%) and for PCA-1 in 24 (45%). In 41 (80%) cases, HRS cells expressed more than one B-cell marker. We then subclassified cases into those positive for plasma cell markers (n = 27) (group 1) and those negative for them (n = 24) (group 2). The average age in group 1 (40 years) was younger than in group 2 (54 years) (P < 0.05). The percentage of nodular sclerosis (NS) subtype in group 1 (52%) was 1.5 times greater than in group 2 (33%) (P < 0.05). With regard to Epstein-Barr virus encoded small RNA (EBER) in-situ hybridization, 14 cases (64%) were positive in group 2, but only seven cases (31%) were positive in group 1 (P < 0.025).
In most cases of classic Hodgkin's disease, HRS cells expressed later stage of B-cell development. We consider that two different clinicopathological groups may correlate with the two different expressions of B-cell markers.
近期分析表明，大多数经典型霍奇金淋巴瘤病例中的霍奇金或里德-斯腾伯格（HRS）细胞源自生发中心B细胞。然而，关于HRS细胞中表达哪些B细胞抗原存在争议。
我们研究了51例经典型霍奇金淋巴瘤病例，采用免疫组织化学方法对HRS细胞进行全B细胞标志物及浆细胞特异性标志物的特征分析。HRS细胞中，18例（35%）表达CD20（L26），9例（18%）表达CD19（B4），13例（25%）表达CD79a（mb-1）。此外，24例（45%）的HRS细胞CD138（B-B4）呈阳性，24例（45%）的PCA-1呈阳性。41例（80%）病例中，HRS细胞表达一种以上B细胞标志物。然后我们将病例分为浆细胞标志物阳性组（n = 27）（第1组）和浆细胞标志物阴性组（n = 24）（第2组）。第1组的平均年龄（40岁）低于第2组（54岁）（P < 0.05）。第1组中结节硬化（NS）亚型的比例（52%）是第2组（33%）的1.5倍（P < 0.05）。关于爱泼斯坦-巴尔病毒编码小RNA（EBER）原位杂交，第2组中有14例（64%）呈阳性，而第1组中仅7例（31%）呈阳性（P < 0.025）。
在大多数经典型霍奇金淋巴瘤病例中，HRS细胞表达B细胞发育的后期阶段。我们认为两种不同的临床病理组可能与B细胞标志物的两种不同表达相关。
