含UGA的支原体基因在枯草芽孢杆菌中的表达。
Expression of UGA-containing Mycoplasma genes in Bacillus subtilis.
作者信息
Kannan T R, Baseman J B
机构信息
Department of Microbiology, University of Texas Health Science Center at San Antonio, San Antonio, Texas 78284-7758, USA.
出版信息
J Bacteriol. 2000 May;182(9):2664-7. doi: 10.1128/JB.182.9.2664-2667.2000.
Abstract
We used Bacillus subtilis to express UGA-containing Mycoplasma genes encoding the P30 adhesin (one UGA) of Mycoplasma pneumoniae and methionine sulfoxide reductase (two UGAs) of Mycoplasma genitalium. Due to natural UGA suppression, these Mycoplasma genes were expressed as full-length protein products, but at relatively low efficiency, in recombinant wild-type Bacillus. The B. subtilis-expressed Mycoplasma proteins appeared as single bands and not as multiple bands compared to expression in recombinant Escherichia coli. Bacillus mutants carrying mutations in the structural gene (prfB) for release factor 2 markedly enhanced the level of readthrough of UGA-containing Mycoplasma genes.
摘要
我们利用枯草芽孢杆菌来表达含有UGA的支原体基因，这些基因编码肺炎支原体的P30黏附素（一个UGA）和生殖支原体的甲硫氨酸亚砜还原酶（两个UGA）。由于天然的UGA抑制作用，这些支原体基因在重组野生型枯草芽孢杆菌中以全长蛋白质产物的形式表达，但效率相对较低。与在重组大肠杆菌中的表达相比，枯草芽孢杆菌表达的支原体蛋白呈现为单一条带而非多条带。在释放因子2的结构基因（prfB）中携带突变的枯草芽孢杆菌突变体显著提高了含有UGA的支原体基因的通读水平。
相似文献
1
Expression of UGA-containing Mycoplasma genes in Bacillus subtilis.含UGA的支原体基因在枯草芽孢杆菌中的表达。
J Bacteriol. 2000 May;182(9):2664-7. doi: 10.1128/JB.182.9.2664-2667.2000.
2
Enhanced readthrough of opal (UGA) stop codons and production of Mycoplasma pneumoniae P1 epitopes in Escherichia coli.增强对乳白（UGA）终止密码子的通读以及在大肠杆菌中产生肺炎支原体P1表位。
Gene. 1993 Nov 30;134(1):33-40. doi: 10.1016/0378-1119(93)90171-x.
3
Molecular cloning and characterization of an adherence-related operon of Mycoplasma genitalium.生殖支原体粘附相关操纵子的分子克隆与特性分析
J Bacteriol. 1995 Oct;177(20):5943-51. doi: 10.1128/jb.177.20.5943-5951.1995.
4
Isolation and characterization of transposon Tn4001-generated, cytadherence-deficient transformants of Mycoplasma pneumoniae and Mycoplasma genitalium.肺炎支原体和生殖支原体中转座子Tn4001产生的细胞黏附缺陷转化体的分离与鉴定
FEMS Immunol Med Microbiol. 1996 Oct;15(4):199-211. doi: 10.1111/j.1574-695X.1996.tb00086.x.
5
Expression and immunological characterization of the carboxy-terminal region of the P1 adhesin protein of Mycoplasma pneumoniae.肺炎支原体P1黏附蛋白羧基末端区域的表达及免疫学特性
J Clin Microbiol. 2005 Jan;43(1):321-5. doi: 10.1128/JCM.43.1.321-325.2005.
6
Cross-hybridization between the cytadhesin genes of Mycoplasma pneumoniae and Mycoplasma genitalium and genomic DNA of Mycoplasma gallisepticum.肺炎支原体与生殖支原体的细胞黏附素基因之间以及鸡毒支原体基因组DNA之间的交叉杂交。
Microb Pathog. 1990 May;8(5):371-5. doi: 10.1016/0882-4010(90)90096-9.
7
Lack of peptide-release activity responding to codon UGA in Mycoplasma capricolum.山羊支原体中缺乏对密码子UGA作出反应的肽释放活性。
Nucleic Acids Res. 1993 Mar 25;21(6):1335-8. doi: 10.1093/nar/21.6.1335.
8
Homologous regions shared by adhesin genes of Mycoplasma pneumoniae and Mycoplasma genitalium.肺炎支原体和生殖支原体黏附素基因共享的同源区域。
Microb Pathog. 1989 Jan;6(1):69-73. doi: 10.1016/0882-4010(89)90009-0.
9
Conditionally lethal and recessive UGA-suppressor mutations in the prfB gene encoding peptide chain release factor 2 of Escherichia coli.大肠杆菌中编码肽链释放因子2的prfB基因的条件致死性和隐性UGA抑制突变。
J Bacteriol. 1988 Nov;170(11):5378-81. doi: 10.1128/jb.170.11.5378-5381.1988.
10
Molecular characterization of the Mycoplasma gallisepticum pvpA gene which encodes a putative variable cytadhesin protein.编码一种假定的可变细胞黏附蛋白的鸡毒支原体pvpA基因的分子特征分析。
Infect Immun. 2000 Jul;68(7):3956-64. doi: 10.1128/IAI.68.7.3956-3964.2000.
引用本文的文献
1
Reviewing advancement in P30 adhesin protein provides insights for future diagnosis and treatment.回顾P30黏附蛋白的研究进展为未来的诊断和治疗提供了思路。
Front Microbiol. 2024 Dec 13;15:1515291. doi: 10.3389/fmicb.2024.1515291. eCollection 2024.
2
Cloning, expression, and immunological characterization of the P30 protein of Mycoplasma pneumoniae.肺炎支原体P30蛋白的克隆、表达及免疫学特性分析
Clin Vaccine Immunol. 2008 Feb;15(2):215-20. doi: 10.1128/CVI.00283-07. Epub 2007 Nov 21.
3
Expression of Mycoplasma proteins carrying an affinity tag in M. pneumoniae allows rapid purification and circumvents problems related to the aberrant genetic code.在肺炎支原体中表达携带亲和标签的支原体蛋白，可实现快速纯化，并避免与异常遗传密码相关的问题。
Appl Environ Microbiol. 2007 Dec;73(23):7799-801. doi: 10.1128/AEM.01861-07. Epub 2007 Oct 12.
4
Multiple-mutation reaction: a method for simultaneous introduction of multiple mutations into the glpK gene of Mycoplasma pneumoniae.多突变反应：一种将多个突变同时引入肺炎支原体glpK基因的方法。
Appl Environ Microbiol. 2005 Jul;71(7):4097-100. doi: 10.1128/AEM.71.7.4097-4100.2005.
5
Determination of infectious load of Mycoplasma genitalium in clinical samples of human vaginal cells.人阴道细胞临床样本中生殖支原体感染负荷的测定
J Clin Microbiol. 2004 Feb;42(2):746-52. doi: 10.1128/JCM.42.2.746-752.2004.
本文引用的文献
1
Disruption of gene mg218 of Mycoplasma genitalium through homologous recombination leads to an adherence-deficient phenotype.通过同源重组破坏生殖支原体的mg218基因会导致粘附缺陷表型。
Proc Natl Acad Sci U S A. 1999 Apr 27;96(9):5227-32. doi: 10.1073/pnas.96.9.5227.
2
Suppression of TGA mutations in the Bacillus subtilis spoIIR gene by prfB mutations.枯草芽孢杆菌spoIIR基因中TGA突变被prfB突变抑制。
J Bacteriol. 1998 Aug;180(16):4166-70. doi: 10.1128/JB.180.16.4166-4170.1998.
3
Mutations in RNAs of both ribosomal subunits cause defects in translation termination.两个核糖体亚基的RNA发生突变会导致翻译终止缺陷。
EMBO J. 1998 Mar 2;17(5):1507-14. doi: 10.1093/emboj/17.5.1507.
4
Stress activation of Bacillus subtilis sigma B can occur in the absence of the sigma B negative regulator RsbX.枯草芽孢杆菌σB的应激激活可在不存在σB负调控因子RsbX的情况下发生。
J Bacteriol. 1997 Mar;179(6):1980-4. doi: 10.1128/jb.179.6.1980-1984.1997.
5
Molecular biology of mycoplasmas.支原体的分子生物学
Annu Rev Microbiol. 1996;50:25-57. doi: 10.1146/annurev.micro.50.1.25.
6
Biofunctional domains of the Mycoplasma pneumoniae P30 adhesin.肺炎支原体P30黏附素的生物功能结构域。
Infect Immun. 1996 Jul;64(7):2595-601. doi: 10.1128/iai.64.7.2595-2601.1996.
7
UGA suppression by a mutant RNA of the large ribosomal subunit.大亚基突变RNA对UGA的抑制作用。
Proc Natl Acad Sci U S A. 1995 Dec 19;92(26):12309-13. doi: 10.1073/pnas.92.26.12309.
8
Enhanced readthrough of opal (UGA) stop codons and production of Mycoplasma pneumoniae P1 epitopes in Escherichia coli.增强对乳白（UGA）终止密码子的通读以及在大肠杆菌中产生肺炎支原体P1表位。
Gene. 1993 Nov 30;134(1):33-40. doi: 10.1016/0378-1119(93)90171-x.
9
The minimal gene complement of Mycoplasma genitalium.生殖支原体的最小基因互补群。
Science. 1995 Oct 20;270(5235):397-403. doi: 10.1126/science.270.5235.397.
10
Molecular basis for cytadsorption of Mycoplasma pneumoniae.肺炎支原体细胞吸附的分子基础。
J Bacteriol. 1982 Sep;151(3):1514-22. doi: 10.1128/jb.151.3.1514-1522.1982.
Zotero 插件