含UGA的支原体基因在枯草芽孢杆菌中的表达。
Expression of UGA-containing Mycoplasma genes in Bacillus subtilis.
作者信息
Kannan T R, Baseman J B
机构信息
Department of Microbiology, University of Texas Health Science Center at San Antonio, San Antonio, Texas 78284-7758, USA.
出版信息
J Bacteriol. 2000 May;182(9):2664-7. doi: 10.1128/JB.182.9.2664-2667.2000.
Abstract
We used Bacillus subtilis to express UGA-containing Mycoplasma genes encoding the P30 adhesin (one UGA) of Mycoplasma pneumoniae and methionine sulfoxide reductase (two UGAs) of Mycoplasma genitalium. Due to natural UGA suppression, these Mycoplasma genes were expressed as full-length protein products, but at relatively low efficiency, in recombinant wild-type Bacillus. The B. subtilis-expressed Mycoplasma proteins appeared as single bands and not as multiple bands compared to expression in recombinant Escherichia coli. Bacillus mutants carrying mutations in the structural gene (prfB) for release factor 2 markedly enhanced the level of readthrough of UGA-containing Mycoplasma genes.
摘要
我们利用枯草芽孢杆菌来表达含有UGA的支原体基因，这些基因编码肺炎支原体的P30黏附素（一个UGA）和生殖支原体的甲硫氨酸亚砜还原酶（两个UGA）。由于天然的UGA抑制作用，这些支原体基因在重组野生型枯草芽孢杆菌中以全长蛋白质产物的形式表达，但效率相对较低。与在重组大肠杆菌中的表达相比，枯草芽孢杆菌表达的支原体蛋白呈现为单一条带而非多条带。在释放因子2的结构基因（prfB）中携带突变的枯草芽孢杆菌突变体显著提高了含有UGA的支原体基因的通读水平。
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