生殖支原体的最小基因互补群。

The minimal gene complement of Mycoplasma genitalium.

作者信息

Fraser C M, Gocayne J D, White O, Adams M D, Clayton R A, Fleischmann R D, Bult C J, Kerlavage A R, Sutton G, Kelley J M, Fritchman R D, Weidman J F, Small K V, Sandusky M, Fuhrmann J, Nguyen D, Utterback T R, Saudek D M, Phillips C A, Merrick J M, Tomb J F, Dougherty B A, Bott K F, Hu P C, Lucier T S, Peterson S N, Smith H O, Hutchison C A, Venter J C

机构信息

Institute for Genomic Research, Rockville, MD 20850, USA.

出版信息

Science. 1995 Oct 20;270(5235):397-403. doi: 10.1126/science.270.5235.397.

DOI:10.1126/science.270.5235.397

PMID:7569993

Abstract

The complete nucleotide sequence (580,070 base pairs) of the Mycoplasma genitalium genome, the smallest known genome of any free-living organism, has been determined by whole-genome random sequencing and assembly. A total of only 470 predicted coding regions were identified that include genes required for DNA replication, transcription and translation, DNA repair, cellular transport, and energy metabolism. Comparison of this genome to that of Haemophilus influenzae suggests that differences in genome content are reflected as profound differences in physiology and metabolic capacity between these two organisms.

摘要

生殖支原体基因组是已知最小的自由生活生物体基因组，其完整核苷酸序列（580,070个碱基对）已通过全基因组随机测序和组装确定。总共仅鉴定出470个预测的编码区域，其中包括DNA复制、转录和翻译、DNA修复、细胞转运及能量代谢所需的基因。将该基因组与流感嗜血杆菌的基因组进行比较表明，基因组内容的差异反映为这两种生物体在生理学和代谢能力上的巨大差异。