大肠杆菌中编码肽链释放因子2的prfB基因的条件致死性和隐性UGA抑制突变。
Conditionally lethal and recessive UGA-suppressor mutations in the prfB gene encoding peptide chain release factor 2 of Escherichia coli.
作者信息
Kawakami K, Inada T, Nakamura Y
机构信息
Department of Tumor Biology, University of Tokyo, Japan.
出版信息
J Bacteriol. 1988 Nov;170(11):5378-81. doi: 10.1128/jb.170.11.5378-5381.1988.
Abstract
Strains carrying mutations in the prfB gene encoding peptide chain release factor 2 of Escherichia coli were isolated. prfB1, prfB2, and prfB3 were selected as suppressor mutations of a lacZ (UGA) mutation at 37 degrees C, one of which, prfB2, is temperature sensitive in growth. A prfB286 strain was selected as a conditionally lethal mutant which grows at 32 but not at 43 degrees C and was shown to have UGA-suppressor activity. All the mutations are recessive UGA-suppressors. These data indicate that release factor 2 is essential to E. coli growth and that all mutants isolated here trigger suppression of the UGA codon.
摘要
分离出了携带编码大肠杆菌肽链释放因子2的prfB基因突变的菌株。prfB1、prfB2和prfB3被选为37℃时lacZ（UGA）突变的抑制突变，其中之一prfB2在生长方面对温度敏感。prfB286菌株被选为条件致死突变体，它在32℃能生长，但在43℃不能生长，并且显示出UGA抑制活性。所有这些突变都是隐性UGA抑制子。这些数据表明释放因子2对大肠杆菌的生长至关重要，并且这里分离出的所有突变体都引发了对UGA密码子的抑制。
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