Cytokine expression by cultured osteoblasts from patients with osteoporotic fractures.
作者信息
Walsh C A, Birch M A, Fraser W D, Ginty A F, Gallagher J A
机构信息
The Bone Research Group, Liverpool Hope University College, Hope Park, Liverpool, UK.
出版信息
Int J Exp Pathol. 2000 Apr;81(2):159-63. doi: 10.1046/j.1365-2613.2000.00147.x.
Abstract
Human osteoblasts were derived in culture from explants of bone from patients who had recently suffered osteoporotic fractures and from patients with no evidence of osteoporosis. The expression of cytokine mRNA in these osteoblasts was subsequently determined by reverse transcriptase-linked polymerase chain reaction (RT-PCR). We have detected mRNA for IL-1beta, IL-3, IL-6, IL-8, TNF-alpha and -beta, and the three TGF-beta isoforms in the cells. The profile of cytokines expressed by osteoblasts derived from patients with osteoporotic fractures was consistent with profiles observed in osteoblasts derived from patients with no evidence of reduced bone mass--the latter included children undergoing corrective surgery and adult subjects ranging from 31 to 80 years undergoing elective surgery for osteoarthritis and other bone pathologies.
摘要
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本文引用的文献
1
Isolation and culture of bone-forming cells (osteoblasts) from human bone.
Methods Mol Med. 1996;2:233-62. doi: 10.1385/0-89603-335-X:233.
2
Mice lacking the type I interleukin-1 receptor do not lose bone mass after ovariectomy.
Endocrinology. 1998 Jun;139(6):3022-5. doi: 10.1210/endo.139.6.6128.
3
Increase in cytokine production (IL-1 beta, IL-6, TNF-alpha but not IFN-gamma, GM-CSF or LIF) by stimulated whole blood cells in postmenopausal osteoporosis.
Maturitas. 1997 Jan;26(1):63-71. doi: 10.1016/s0378-5122(96)01080-8.
4
Estrogen, cytokines, and pathogenesis of postmenopausal osteoporosis.
J Bone Miner Res. 1996 Aug;11(8):1043-51. doi: 10.1002/jbmr.5650110802.
5
In vitro production of cytokines by bone surface-derived osteoblastic cells in normal and osteoporotic postmenopausal women: relationship with cell proliferation.
J Clin Endocrinol Metab. 1993 Sep;77(3):824-30. doi: 10.1210/jcem.77.3.8370705.
6
PCR detection of cytokines in normal human and pagetic osteoblast-like cells.
J Bone Miner Res. 1993 Oct;8(10):1155-62. doi: 10.1002/jbmr.5650081002.
7
Analysis of gene expression in human bone biopsies by polymerase chain reaction: evidence for enhanced cytokine expression in postmenopausal osteoporosis.
J Bone Miner Res. 1994 Jun;9(6):883-90. doi: 10.1002/jbmr.5650090614.
8
Primary cultures of human bone-derived cells produce parathyroid hormone-related protein: a study of 40 patients of varying age and pathology.
Bone Miner. 1994 Oct;27(1):43-50. doi: 10.1016/s0169-6009(08)80185-7.
9
An interleukin 1 like factor stimulates bone resorption in vitro.
Nature. 1983;306(5941):378-80. doi: 10.1038/306378a0.
10
Stimulation of the proliferation of human bone cells in vitro by human monocyte products with interleukin-1 activity.
J Clin Invest. 1985 Apr;75(4):1223-9. doi: 10.1172/JCI111819.
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