Walsh C A, Birch M A, Fraser W D, Robinson J, Lawton R, Dorgan J, Klenerman L, Gallagher J A
University Department of Clinical Chemistry, Royal Liverpool University Hospital, UK.
Bone Miner. 1994 Oct;27(1):43-50. doi: 10.1016/s0169-6009(08)80185-7.
Parathyroid hormone-related protein (PTHrP), a mediator of hypercalcemia of malignancy, has been detected in many tumours and in some normal foetal and adult tissues. PTHrP has potent effects on bone turnover in vivo and in vitro. In this study we cultured cells derived from explants of bone obtained from 40 subjects (age range 2-88 years). Immunoreactive PTHrP (iPTHrP) was detected by immunoradiometric assay (IRMA) in conditioned medium from 25 of 40 cultures of bone-derived cells. PTHrP mRNA was detected in bone-derived cells by reverse transcriptase-linked polymerase chain reaction (RT-PCR). The identity of PCR products was confirmed by Southern blotting. Local production of PTHrP in vivo may be important in the regulation of bone growth and remodelling.
甲状旁腺激素相关蛋白（PTHrP）是恶性肿瘤高钙血症的介质，已在许多肿瘤以及一些正常胎儿和成人组织中被检测到。PTHrP在体内和体外对骨转换均有显著影响。在本研究中，我们培养了来自40名受试者（年龄范围2至88岁）骨外植体的细胞。通过免疫放射分析（IRMA）在40份骨源性细胞培养物中的25份的条件培养基中检测到免疫反应性PTHrP（iPTHrP）。通过逆转录 - 聚合酶链反应（RT-PCR）在骨源性细胞中检测到PTHrP mRNA。PCR产物的同一性通过Southern印迹法得到证实。PTHrP在体内的局部产生可能在骨生长和重塑的调节中起重要作用。
