Lorenzo J A, Naprta A, Rao Y, Alander C, Glaccum M, Widmer M, Gronowicz G, Kalinowski J, Pilbeam C C
VA Connecticut Healthcare System, Newington 06111, USA.
Endocrinology. 1998 Jun;139(6):3022-5. doi: 10.1210/endo.139.6.6128.
We measured the effects of ovariectomy on the bone mass of mice that lacked type I interleukin-1 receptor (IL-I R1 -/- mice) in two genetic backgrounds (C57BL/6 x 129/Sv and C57BL/6) to investigate the role of interleukin-1 in the actions of estrogen on bone. At three weeks after surgery, ovariectomized wild-type mice decreased trabecular bone volume in the proximal humerus by 70% in a C57BL/6 x 129/Sv background and 48% in a C57BL/6 background compared to sham-operated controls. In contrast, there was no significant decrease in trabecular bone mass in IL-1 R1 -/- mice after ovariectomy. The estrogen status of all groups was confirmed by measurement of uterine wet weight. These results demonstrate that a functional IL-1 response pathway is required for mice to lose trabecular bone mass after ovariectomy in this model and they imply that IL-1 is an important mediator of the effects of ovariectomy on bone mass. Hence, therapeutic interventions that block the effects of IL-1 on bone may be beneficial for treating diseases of rapid bone loss such as post-menopausal osteoporosis.
我们在两种遗传背景（C57BL/6×129/Sv和C57BL/6）下，测量了卵巢切除对缺乏I型白细胞介素-1受体的小鼠（IL-1 R1 -/-小鼠）骨量的影响，以研究白细胞介素-1在雌激素对骨骼作用中的角色。术后三周，在C57BL/6×129/Sv背景下，卵巢切除的野生型小鼠肱骨近端小梁骨体积比假手术对照组减少了70%；在C57BL/6背景下，减少了48%。相比之下，卵巢切除后IL-1 R1 -/-小鼠的小梁骨量没有显著减少。通过测量子宫湿重确认了所有组的雌激素状态。这些结果表明，在该模型中，功能性IL-1反应途径是小鼠卵巢切除后小梁骨量减少所必需的，这意味着IL-1是卵巢切除对骨量影响的重要介质。因此，阻断IL-1对骨骼影响的治疗干预可能有益于治疗如绝经后骨质疏松症等快速骨质流失疾病。
