一种从红细胞中洗脱抗体的新方法。

A new method of antibody elution from red blood cells.

作者信息

Chan-Shu S A, Blair O

出版信息

Transfusion. 1979 Mar-Apr;19(2):182-5. doi: 10.1046/j.1537-2995.1979.19279160290.x.

DOI:10.1046/j.1537-2995.1979.19279160290.x

Abstract

A method of antibody elution from red blood cells using xylene is described. It can be completed in 15 minutes after cell washing and requires no special equipment. For warm antibodies, the eluates prepared were more potent than those prepared by Rubin's ether method. It was as effective as Landsteiner and Miller's heat elution method in investigations of ABO hemolytic disease of the newborn. The clinical application of this method in the investigation of hemolytic disease of the newborn, delayed transfusion reaction and autoimmune hemolytic anemia was described.

摘要

描述了一种使用二甲苯从红细胞中洗脱抗体的方法。细胞洗涤后15分钟即可完成，无需特殊设备。对于温抗体，所制备的洗脱液比用鲁宾乙醚法制备的更有效。在新生儿ABO溶血病的研究中，其效果与兰德施泰纳和米勒的热洗脱法相同。描述了该方法在新生儿溶血病、迟发性输血反应和自身免疫性溶血性贫血研究中的临床应用。