Almeida A P, Da Silva S A, Souza M L, Lima L M, Rossi-Bergmann B, de Moraes V L, Costa S S
Nucleo de Pesquisas de Produtos Naturais, Universidade Federal do Rio de Janeiro, Brazil.
Planta Med. 2000 Mar;66(2):134-7. doi: 10.1055/s-2000-11131.
Previously we demonstrated that Kalanchoe pinnata (KP) leaf extracts inhibited in vitro lymphocyte proliferation and showed in vivo immunosuppressive activity. Here we attempt to identify the immunosuppressive substances present in KP guided by the lymphoproliferative assays. From the ethanolic extract was purified a fraction (KP12SA) twenty-fold more potent to block murine lymphocyte proliferation than the crude extract. Chemical analysis by 1H- and 13C-NMR, IR and GC-MS of KP12SA (methylated sample) showed 89.3% of palmitic acid (C16), 10.7% of stearic acid (C18) and traces of arachidic (C20) and behenic acids (C22). This study provides evidence that fatty acids present in Kalanchoe pinnata may be responsible, at least in part, for its immunosuppressive effect in vivo.
此前我们证明，落地生根（KP）叶提取物在体外可抑制淋巴细胞增殖，并在体内表现出免疫抑制活性。在此，我们试图通过淋巴细胞增殖试验来鉴定KP中存在的免疫抑制物质。从乙醇提取物中纯化出一种组分（KP12SA），其阻断小鼠淋巴细胞增殖的效力比粗提物高20倍。通过对KP12SA（甲基化样品）进行1H-和13C-NMR、IR和GC-MS化学分析，结果显示含有89.3%的棕榈酸（C16）、10.7%的硬脂酸（C18）以及痕量的花生酸（C20）和山嵛酸（C22）。本研究提供了证据，表明落地生根中存在的脂肪酸可能至少部分地是其在体内产生免疫抑制作用的原因。
