Coutinho Marcela A S, Muzitano Michelle F, Cruz Elaine A, Bergonzi Maria C, Kaiser Carlos R, Tinoco Luzineide W, Bilia Anna R, Vincieric Franco F, Rossi-Bergmann Bartira, Costa Sônia S
Núcleo de Pesquisas de Produtos Naturais, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
Nat Prod Commun. 2012 Feb;7(2):175-8.
The chemical composition and immunosuppressive potential of the flowers from Kalanchoe pinnata (Crassulaceae) were investigated. We found that the aqueous flower extract was more active than the leaf extract in inhibiting murine T cell mitogenesis in vitro. Flavonoids isolated from the flower extract were identified and quantitated based on NMR and HPLC-DAD-MS analysis, respectively. Along with quercetin, four quercetin glycosyl conjugates were obtained, including quercetin 3-O-beta-D-glucuronopyranoside and quercetin 3-O-beta-D-glucopyranoside, which are described for the first time in K. pinnata. All flavonoids inhibited murine T cell mitogenesis and IL-2 and IL-4 production without cell toxicity. This is the first report on the pharmacological activity of flowers of a Kalanchoe species, which are not used for curative purposes. Our findings show that K. pinnata flowers are a rich source of T-suppressive flavonoids that may be therapeutically useful against inflammatory diseases.
对落地生根（景天科）花朵的化学成分和免疫抑制潜力进行了研究。我们发现，花的水提取物在体外抑制小鼠T细胞有丝分裂方面比叶提取物更具活性。分别基于核磁共振（NMR）和高效液相色谱-二极管阵列-质谱（HPLC-DAD-MS）分析对从花提取物中分离出的黄酮类化合物进行了鉴定和定量。除槲皮素外，还获得了四种槲皮素糖基共轭物，包括槲皮素3-O-β-D-葡萄糖醛酸吡喃糖苷和槲皮素3-O-β-D-葡萄糖吡喃糖苷，这在落地生根中首次被描述。所有黄酮类化合物均抑制小鼠T细胞有丝分裂以及白细胞介素-2（IL-2）和白细胞介素-4（IL-4）的产生，且无细胞毒性。这是关于一种未用于治疗目的的落地生根属植物花朵药理活性的首次报道。我们的研究结果表明，落地生根花是T抑制性黄酮类化合物的丰富来源，可能对炎症性疾病具有治疗作用。
