类胡萝卜素对培养的大鼠腹水肝癌细胞侵袭的抑制作用。

Inhibitory effects of carotenoids on the invasion of rat ascites hepatoma cells in culture.

作者信息

Kozuki Y, Miura Y, Yagasaki K

机构信息

Department of Applied Biological Science, Tokyo Noko University, Fuchu, Japan.

出版信息

Cancer Lett. 2000 Apr 3;151(1):111-5. doi: 10.1016/s0304-3835(99)00418-8.

DOI:10.1016/s0304-3835(99)00418-8

PMID:10766430

Abstract

The effects of carotenoids--alpha-carotene, beta-carotene, lycopene, beta-cryptoxanthin, zeaxanthin, lutein, canthaxanthin, astaxanthin--on the invasion of rat ascites hepatoma AH109A cells were investigated by co-culturing the hepatoma cells with rat mesentery-derived mesothelial cells (M-cells). All the carotenoids examined inhibited AH109A invasion in a dose-dependent manner up to 5 microM. Cancer cells previously cultured with hypoxanthine (HX) and xanthine oxidase (XO) showed a highly invasive activity. Carotenoids, 5 microM of beta-carotene and astaxanthin, suppressed this reactive oxygen species-potentiated invasive capacity by simultaneously treating AH109A cells with the carotenoids, HX and XO. These results suggest that the antioxidative property of these carotenoids may be involved in their anti-invasive action.

摘要

通过将大鼠腹水肝癌AH109A细胞与大鼠肠系膜来源的间皮细胞（M细胞）共培养，研究了类胡萝卜素——α-胡萝卜素、β-胡萝卜素、番茄红素、β-隐黄质、玉米黄质、叶黄素、角黄素、虾青素——对大鼠腹水肝癌AH109A细胞侵袭的影响。所有检测的类胡萝卜素在高达5微摩尔的浓度下均以剂量依赖方式抑制AH109A细胞的侵袭。先前用次黄嘌呤（HX）和黄嘌呤氧化酶（XO）培养的癌细胞表现出高度侵袭活性。5微摩尔的β-胡萝卜素和虾青素等类胡萝卜素，通过同时用类胡萝卜素、HX和XO处理AH109A细胞，抑制了这种活性氧增强的侵袭能力。这些结果表明，这些类胡萝卜素的抗氧化特性可能与其抗侵袭作用有关。