Department of Applied Biological Science, Tokyo Noko University, Saiwai-cho 3-5-8, Fuchu, Tokyo, 183-8509, Japan.
Cytotechnology. 2005 Jan;47(1-3):133-8. doi: 10.1007/s10616-005-3750-y.
Effect of ascorbic acid (AsA) on the proliferation and invasion of rat ascites hepatoma AH109A cells was investigated by measuring [(3)H]thymidine incorporation into acid-insoluble fraction of the cells and by co-culturing the hepatoma cells with rat mesentery-derived mesothelial cells, respectively. AsA suppressed the invasion of AH109A cells in a dose-dependent manner at concentrations of 62.5-500 muM, while it inhibited the proliferation of the cells at higher concentrations of 250 and 500 muM. Hepatoma cells previously cultured with hypoxanthine (HX) and xanthine oxidase (XO) or with hydrogen peroxide showed increased invasive activities. AsA suppressed the reactive oxygen species-potentiated invasive capacity by simultaneously treating AH109A cells with AsA, HX and XO or with AsA and hydrogen peroxide. Furthermore, AsA reduced the intracellular peroxide levels in AH109A cells. These results suggest that the antioxidative property of AsA may be involved in its anti-invasive action on hepatoma cells.
研究了抗坏血酸（AsA）对大鼠腹水肝癌 AH109A 细胞增殖和侵袭的影响，方法是分别测量细胞内[（3）H]胸苷掺入酸不溶性部分和将肝癌细胞与大鼠肠系膜衍生间皮细胞共培养。AsA 以 62.5-500μM 的浓度剂量依赖性地抑制 AH109A 细胞的侵袭，而在更高浓度 250 和 500μM 时抑制细胞增殖。先前用次黄嘌呤（HX）和黄嘌呤氧化酶（XO）或用过氧化氢培养的肝癌细胞显示出侵袭活性增加。AsA 通过同时用 AsA、HX 和 XO 或用 AsA 和过氧化氢处理 AH109A 细胞来抑制活性氧增强的侵袭能力。此外，AsA 降低了 AH109A 细胞内的过氧化物水平。这些结果表明，AsA 的抗氧化特性可能与其对肝癌细胞的抗侵袭作用有关。
