Wienecke R, Wolff H, Schaller M, Meyer H, Plewig G
Department of Dermatology and Allergology, Klinikum Innenstadt, Ludwig-Maximilians-University Munich, Munich, Germany.
J Am Acad Dermatol. 2000 May;42(5 Pt 2):892-4. doi: 10.1016/s0190-9622(00)90265-2.
We describe an 11-year-old girl with a cowpox virus infection, who presented with a 14-day-old crusted, ulcerated nodule on the chin/neck and a 6-day-old eroded blister on the left leg. The girl lived in a rural environment, had close contact to several cats from the neighborhood, and had an atopic predisposition. The presence of orthopox virus in the lesion on the left leg was demonstrated by electron microscopy (negative staining, transmission electron microscopy) and virus isolation. Classification as a cowpox virus was determined by polymerase chain reaction (PCR), followed by restriction enzyme digestion of the PCR product.
我们描述了一名患有牛痘病毒感染的11岁女孩，她的下巴/颈部有一个14天的结痂、溃疡结节，左腿有一个6天的糜烂水疱。该女孩生活在农村环境中，与邻居的几只猫有密切接触，并有特应性易感性。通过电子显微镜（负染色、透射电子显微镜）和病毒分离证实左腿病变中存在正痘病毒。通过聚合酶链反应（PCR）确定为牛痘病毒，随后对PCR产物进行限制性酶切消化。
