Jin W, Xu Q, Li W
Laboratory of Analytical Science, School of Chemistry, Shandong University, Jinan, China.
Electrophoresis. 2000 May;21(8):1527-34. doi: 10.1002/(SICI)1522-2683(20000501)21:8<1527::AID-ELPS1527>3.0.CO;2-Z.
A self-aligning end-column amperometric detection system for capillary electrophoresis was constructed. In this system, the electrode and capillary were exchanged easily and the capillary/electrode alignment procedure is not required. Gold, gold/mercury amalgam, copper and carbon fiber could be used as the working electrode. The principle is in the use of two disk holders with the capillary and the electrode in the center, so that by inserting the disk holders into a groove in the working electrode port, the capillary and the electrode are automatically aligned and the distance between the capillary and the electrode is assured at 0.24 mm. The relative standard deviation obtained using five different gold/mercury amalgam microdisk electrodes for determination of cysteine was 1.5% for the migration time and 3.3% for the electrophoretic peak current. The simple and convenient system was attractive for the routine analysis by capillary electrophoresis with electrochemical detection. The system was applied to the determination of promethazine hydrochloride in human serum.
构建了一种用于毛细管电泳的自对准末端柱安培检测系统。在该系统中，电极和毛细管易于更换，无需进行毛细管/电极对准程序。金、金/汞齐、铜和碳纤维均可用作工作电极。其原理是使用两个圆盘支架，毛细管和电极位于中心，通过将圆盘支架插入工作电极端口的凹槽中，毛细管和电极自动对准，毛细管与电极之间的距离确保为0.24毫米。使用五个不同的金/汞齐微盘电极测定半胱氨酸时，迁移时间的相对标准偏差为1.5%，电泳峰电流的相对标准偏差为3.3%。该简单便捷的系统对于毛细管电泳电化学检测的常规分析具有吸引力。该系统应用于测定人血清中的盐酸异丙嗪。
