对来自巴西的苏云金芽孢杆菌分离株进行鞘翅目特异性cry基因存在情况的筛选与鉴定。

Screening and characterization of Bacillus thuringiensis isolates from Brazil for the presence of coleoptera-specific cry genes.

作者信息

Marquez A M, Dias J M, Ribeiro B M

机构信息

Departmento de Biologia Celular, Instituto de Ciências Biológicas, Universidade de Brasília, Brazil.

出版信息

Microbiol Res. 2000 Mar;154(4):355-62. doi: 10.1016/S0944-5013(00)80010-5.

DOI:10.1016/S0944-5013(00)80010-5

PMID:10772158

Abstract

Forty-three Bacillus thuringiensis isolates from Brazil and 3 from Argentina were screened, using the polymerase chain reaction (PCR), for various coleoptera-specific cry genes. Seven isolates produced specific and/or nonspecific DNA fragments in a PCR reaction with primers specific for two coleopteran cry genes and 4 of these produced DNA fragments with primers specific for 7 known coleopteran cry genes. These isolates showed, by electron microscopy, the presence of spherical crystals. They also showed proteins of around 70 kDa which were immunologically similar to the Cry3Aa protein from B. thuringiensis subsp. tenebrionis. The 3 isolates from Argentina were toxic to T. molitor, and although no isolate from Brazil showed toxicity, they might show toxicity to another insect species.

摘要

利用聚合酶链反应（PCR）对来自巴西的43株苏云金芽孢杆菌分离株和来自阿根廷的3株分离株进行了各种鞘翅目特异性cry基因的筛选。7株分离株在与两种鞘翅目cry基因特异性引物的PCR反应中产生了特异性和/或非特异性DNA片段，其中4株与7种已知鞘翅目cry基因特异性引物产生了DNA片段。通过电子显微镜观察，这些分离株呈现出球形晶体。它们还显示出约70 kDa的蛋白质，这些蛋白质在免疫上与苏云金芽孢杆菌亚种tenebrionis的Cry3Aa蛋白相似。来自阿根廷的3株分离株对黄粉虫有毒，尽管来自巴西的分离株均未显示出毒性，但它们可能对其他昆虫物种具有毒性。