Hongyu Z, Ziniu Y, Wangxi D
Department of Plant Protection, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
J Invertebr Pathol. 2000 Oct;76(3):191-7. doi: 10.1006/jipa.2000.4970.
The composition and distribution of insecticidal crystal proteins (Cry proteins) and their genotypes of Bacillus thuringiensis isolates from warehouses were evaluated through SDS-PAGE and PCR techniques. The results showed that the electrophoretic patterns of delta-endotoxin crystal preparations were divided into five types. The isolates containing approximately 135 kDa with a 65-kDa protein or only a approximately 135-kDa protein, which amounted to 55.74 and 35.25% of all isolates respectively, were the two major profiles of Cry protein isolated. The distribution of cry genes of B. thuringiensis from warehouses was highly variable. Cry protein genotypes detected in B. thuringiensis isolates included cry1Aa5, cry1Ab9, cry1Ac5, cry1Ba, cry1Ca1, cry1Da1, cry1Ea3, cry2, and cry3 genes, but not cry1Fa2. Among them, cry2, cry1Ac5, and cry1Ab9 genes were the most common in our B. thuringiensis isolates. Most B. thuringiensis isolates contained several cry genes in a total of 18 profiles. Among them, cry1Ac5 with cry1Ea3; cry1Aa5, cry1Ab9, cry1Ac5 with cry1Ea3; and cry1Aa5, cry1Ab9 with cry1Ac5 were the three principal profiles. The distribution of the Cry proteins and cry genes in isolates depended on geography and type of warehouses. Gene profiles may be used as markers for insecticidal activity of B. thuringiensis strains, but they did not directly reflect the toxic level of B. thuringiensis strains. The serotype of B. thuringiensis strains did not directly reflect the specific cry gene profiles in the strains, but certain relationships can be established between the serotype and cry genotype.
通过SDS - PAGE和PCR技术评估了来自仓库的苏云金芽孢杆菌分离株的杀虫晶体蛋白（Cry蛋白）的组成、分布及其基因型。结果表明，δ - 内毒素晶体制剂的电泳图谱分为五种类型。含有约135 kDa和65 kDa蛋白或仅约135 kDa蛋白的分离株分别占所有分离株的55.74%和35.25%，是分离得到的Cry蛋白的两种主要图谱。来自仓库的苏云金芽孢杆菌cry基因的分布高度可变。在苏云金芽孢杆菌分离株中检测到的Cry蛋白基因型包括cry1Aa5、cry1Ab9、cry1Ac5、cry1Ba、cry1Ca1、cry1Da1、cry1Ea3、cry2和cry3基因，但不包括cry1Fa2。其中，cry2、cry1Ac5和cry1Ab9基因在我们的苏云金芽孢杆菌分离株中最为常见。大多数苏云金芽孢杆菌分离株总共含有18种图谱中的几种cry基因。其中，cry1Ac5与cry1Ea3；cry1Aa5、cry1Ab9、cry1Ac5与cry1Ea3；以及cry1Aa5、cry1Ab9与cry1Ac5是三种主要图谱。分离株中Cry蛋白和cry基因的分布取决于地理位置和仓库类型。基因图谱可作为苏云金芽孢杆菌菌株杀虫活性的标记，但它们不能直接反映苏云金芽孢杆菌菌株的毒性水平。苏云金芽孢杆菌菌株的血清型不能直接反映菌株中特定的cry基因图谱，但血清型与cry基因型之间可以建立一定的关系。
